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Description
Human IFN-γ ELISA KitProduct Specification Species Reactivity Human Theory This kit adopts double antibody sandwich enzyme linked immunosorbent detection technology. Specific anti human IFN antibodies were pre coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IFN present in the sample binds to the solid phase antibody and the
Product Specification
| Species Reactivity | Human | |||||||||||||||||||||||||||
| Theory | This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human IFN-γ antibodies were pre-coated on high affinity plates. The standard substance, the sample to be tested and the biotinylated detection antibody are added to the well of the enzyme label plate, and after incubation, the IFN-γ present in the sample binds to the solid phase antibody and the detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, a chromogenic substrate is added to protect the color from light. A stop solution was added to stop the reaction, and the absorbance value was measured at a wavelength of 450 nm (reference calibration wavelength of 540 nm or 570 nm). | |||||||||||||||||||||||||||
| Synonym | Human interferon gamma ELISA kit, IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma | |||||||||||||||||||||||||||
| Composition |
Please use it within the validity period of the kit (new and old products are shipped randomly)
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| Background | Gamma interferon (also known as type II interferon) is an important cytokine that can exercise immunomodulatory function. It was discovered for its antiviral activity. Interferon gamma plays a key role in host defense through antiviral, antiproliferative, and immunomodulatory functions. In many types of cells, gamma interferon induces cytokine production and upregulates the expression of different membrane proteins, including type I/II major histocompatibility complex (MHC-I, MHC-II), Fc receptors, leukocyte adhesion molecules, B7 family antigens. Gamma interferon can effectively activate macrophages and guide the synthesis, type transformation and secretion of B cell immunoglobulin. Gamma interferon can also affect the development of T helper cell subtypes by inhibiting the differentiation of Th2 and stimulating the growth of Th1. Gamma interferon plays an important role in some inflammatory disease processes such as autoimmunity and atherosclerosis. Biologically active gamma interferon is a non-covalently bound dimer with a molecular weight of about 20-25KD and varying degrees of glycosylation. Mature human interferon gamma has close to 90% homology with the amino acid sequence of interferon gamma from rhesus monkey, 59-64% homology with interferon gamma from cattle, dog, horse, cat and pig, and 37-43% homology with interferon gamma from cotton rat, mouse and rat. The interferon dimer first binds to the transmembrane receptor IFN-γRI (α subunit), and then binds to IFN-γRII (β subunit) to form a functional complex containing two α subunits and two β subunits; Wherein the IFN-γRII in the complex receptor can increase the affinity of the ligand as well as the signal transduction efficiency. Although the γ subunit is constitutively expressed in many types of cells, the β subunit cell expression regulates the influence of the response state of the receptor interferon γ and is strictly regulated. Interferon gamma can be secreted by many cells under a series of inflammatory conditions, including dendritic epidermal/γδ T cells, keratinocytes, peripheral blood γδ T cells, mast cells, neurons, CD8 + T cells, macrophages, B cells, neutrophils, natural killer cells, CD4 + T cells and testicular sperm cells, etc. |
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| General Notes | 1. Please use the kit within the validity period. 2. The components of different kits and different batch kits cannot be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with diluent (1 ×) and re-tested; If the cell culture supernatant sample needs to be distributed and diluted, cell culture medium can be used for other intermediate dilutions except dilution with diluent in the last step. 4. Differences in test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipette, the plate washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The terminating solution in the kit is an acidic solution. Please protect your glasses, hands, face and clothes when using it. |
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| Storage Temp. | Kit unopened, stored at 2-8 °C. | |||||||||||||||||||||||||||
| Test Range | 9.38pg/mL-600pg/mL |
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4.4 ★★★★★
Based on 15 reviews
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Product Reviews
★★★★★ 5
Excellent Rivals to Lovers!!
Format: Kindle
The tension and banter between Huntyr and Wold was delectable. I absolutely love the fallen angel and all of his flaws. Huntyr is amazing too being a badass FMC with some major trauma. The world building was great and I enjoyed the training aspect of the story. The writing was immersive and was in the story the whole time. The ending had quite a twist that I hadn’t anticipated and made my jaw DROP. Excellent job! I also loved the narration. Laura is one of my fave narrators!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 15, 2025
★★★★★ 5
Fallen Angels, fae, vampires, oh my!
Format: Kindle
Rating: 4.5 | Spice: 2 (but a good slow-burn)
•
Main Characters: Huntyr and Wolf
•
I couldn’t wait to read this book; there was so much hype about it! And there was no doubt why. I fell in love with the characters and the plot itself. This book is mainly plot driven more than friction driven but it’s easy to follow along with. The characters are fun, easily understood. The main setting is at an academy where both the main characters are going through trials and building strength for the final test, The Transcendent. There are fantastic side characters as well. I loved the camaraderie between Huntyr and her friends. But we don’t like Lanson. 😆
We do have some plot twists that come into play throughout the book. Secrets and betrayal to be seen. I did adore Wolf and Huntyr’s relationship. It was a classic slow burn trope. They didn’t hit it off fast, but in time their feelings grew. I loved their banter, so sexy. Wolf is your next book boyfriend; Huntyr is your next vampire assassin independent bad-a*s female. Themes include loyalty, trust, self-discovery, a true slow burn romance. Side note: book ends on a angsty cliffhanger!
•
Emily, thank you for writing this awesome novel and I cannot wait to devour Book 2, Blood So Brutal! 😍
•
Happy reading, my lovelies! xo
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Reviewed in the United States on January 21, 2024
★★★★★ 4
“My heart bows to you and you only, Huntress.”
Format: Kindle
3.5 🌟
This book popped up in my KU recommended reading suggestions and the synopsis sounded like what I was in the mood for. I'm so glad I took a chance on it. I went into this knowing absolutely nothing about it and ended up really liking it. I love when this happens.
The main characters are likeable and I easily found myself rooting for them. There is a mystery element to each of their backstories that I enjoyed watching unfold and can't wait to get more of. Wolf, in particular, has me fixated. Love him.
I found this to be an entertaining, addictive read with a plot that moves along at a good pace. It reads so easily I found myself very reluctant to put it down. Lots of twists and turns and the angst is there. A good set up for the next book to come, for sure.
My issues with this book....the dialogue feels a bit juvenile at times and there is a repetitive over use of a particular word phrasing that I found myself giving the ole eye-roll to. There are, without a doubt, some pretty cliche moments that gave me a bit of the cringe. I think this could've certainly 100% benefited from more depth regarding the world building. Perhaps the world building was sacrificed to keep the pacing quick? Just a guess. Also, the lack of consistency of character for the FMC was really evident and so she feels quite illogical at times.
Overall, this was a fun and enjoyable read that hit the spot well enough for me. That ending certainly has me impatiently pining for book 2!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 18, 2024
★★★★★ 3
Interesting take on the genre
Format: Kindle
True rating: 3.25 ⭐️
I enjoyed the fresh take on the genre. The best way I could describe the setting and world is an apocalyptic dystopian version of Farie where vampires, fae, and angles struggle to survive in what is left of the world. It was definitely interesting throwing the academy/hunger games aspect into this world as well.
Even though I guessed the final reveal early on in the book, I kept hoping I was wrong, and it would take a surprising turn. While the "plot twists" were a bit predictable to me, I still enjoyed the ride this book took me on.
Another downfall for me was the plot holes in the world building... I.E. if society has fallen and the world is in the aftermath of war, how are there trains running around the world? Just to take young adults to the trials to get into the golden city? How is the train maintained, the tracks clear, etc?
However, I did enjoy the FMC & MMC and thought they were fleshed out nicely. I also enjoyed the side characters but wish some were developed more like Ashalin (sp?). I do find myself rooting for the MCs to succeed and find happiness together, which is obviously an important aspect for romantasy.
Overall, was this an earth-shattering, mind-bending, terrific piece of literature? No. But was it the worst thing I've read this year? Also, no. This book has, to me, the bones of a great read & just needs a bit more to push it from an alright book to a great book.
Overall ratings:
Plot- 3.5⭐️
World building 3⭐️
Spice 2.5 🌶🌶
Main characters 4 ⭐️
Supporting characters 3.5⭐️
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Reviewed in the United States on May 12, 2024
★★★★★ 5
great book
Format: Kindle
I am really excited to meet the author at the book retreat this month. I really enjoyed this world that she built and most of the female main character Huntress is so awesome. She goes through a lot in this book and the ending; wow! I wouldn't have even guessed. I highly recommend everyone to read this book.
I have been so lucky this year that almost all the books I have read have been, so far, 5 out 5 stars.
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Reviewed in the United States on June 2, 2026
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