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Description
Human MRGBP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against MORF4-related gene binding protein (MRGBP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of MORF4-related gene binding protein (MRGBP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human MORF4-related gene binding protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | MORF4-related gene-binding protein (MRGBP) is a subunit of the NuA4 histone acetyltransferase complex, which is primarily involved in the transcriptional activation of specific genes through acetylation of ribosomal histones H4 and H2A. This modification may alter ribosome-DNA interactions and promote interactions of the modified histones with other proteins, thereby actively regulating transcription. This complex may be required for the activation of transcriptional programs involved in oncogene- and proto-oncogene-mediated growth induction, tumor suppressor-mediated growth arrest and replicative senescence, apoptosis, and DNA repair. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 19 reviews
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Product Reviews
★★★★★ 5
Best skincare product ever
Size: 3.38 Fl Oz (Pack of 1)
This ampoule is THE BEST THING EVER. I’ve been in the skincare industry since 1996 and I’ve never used a product that works as well as this. I have sensitive oily skin and it has a lot of redness. After the first use the redness was completely gone and the hydration was noticeable. Do not pass on this stuff! Highly recommend it for anyone wanting immediate hydration. It’s thin and isn’t sticky or tacky. It feels like I don’t have anything on my face but the benefits are definitely noticeable.
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Reviewed in the United States on April 18, 2026
★★★★★ 4
Good product, but requires consistent use
Size: 1.85 Fl Oz (Pack of 1), Size: 1.85 Fl Oz (Pack of 1)
I’ve been using this centella ampoule for a while now, and overall I like it. The texture is lightweight, absorbs quickly, and leaves the skin feeling hydrated and soothed. It’s especially nice for sensitive or irritated skin.
However, one important thing to note is that the results are not immediate. In my experience, you start seeing improvements with consistent, long-term use rather than overnight. If you’re patient and use it daily, it can definitely help improve your skin’s appearance.
Overall, it’s a good product, but it requires consistency to see full results.
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Reviewed in the United States on April 17, 2026
★★★★★ 5
Keeping this in my skincare regimen for the long-haul
Size: 3.38 Fl Oz (Pack of 1)
No matter what skin type you are, you should have this in your lineup. I have larger pores - so sebaceous filaments & clogging. On top of the oiliness of my skin, I have more oily hair as well. But, I get dry patches like crazy. So it’s not easy to find products that meet all of my needs or are universal - it’s usually an “either or” situation. & I end up buying things for my drier days as well as products for my oily days instead of just one set of products. This is one that work well no matter what. There’s more than you’d expect based on the photos - the bottle is bigger. A little goes a long way. It’s very gentle. Great for barrier repair. Great for redness/irritation. A little goes a long way, but it can also be used more than once without being overwhelming. It’s great as a base for other serums/moisturizers, morning or night. It feels really good - clean & light (but not “light” as in ineffective, it still pulls its weight). Must have for anyone looking for trendy Korean glass-skin skincare - she’s one that will get you there without needing to invest in random “viral” products.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
LOVEE (combo & oily skin) 🥹
Size: 3.38 Fl Oz (Pack of 1)
Pretty good! I loveee the applicator since it makes it really easy to apply. My skin has been super glowy ever since I’ve used this. Consistency is super thin and lightweight. My skin doesn’t exactly feel soft but looks really smooth. Despite it being this way, it hydrates super well!
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Reviewed in the United States on March 23, 2026
★★★★★ 5
Gentle Yet Effective—Perfect for Redness and Sensitive Skin
I’ve been really impressed with this Anua Azelaic Acid serum—it’s one of those products that feels gentle but still delivers noticeable results. The formula is lightweight and absorbs quickly, leaving no sticky or heavy feeling behind, which makes it easy to layer with the rest of my skincare.
What stands out most is how soothing it is. The 10% azelaic acid helps calm redness and irritation while still being mild enough for sensitive skin. Combined with multiple types of hyaluronic acid, it keeps my skin hydrated and balanced instead of dry or tight. 
Over time, I noticed my skin tone looking more even and less inflamed, especially in areas prone to breakouts or redness. It’s a great option if you want something that targets multiple concerns—like texture, redness, and hydration—without being too harsh.
Overall, this is a fantastic serum for anyone dealing with sensitive or reactive skin. It’s effective, easy to use, and feels like a high-quality addition to a daily routine. I’ll definitely keep repurchasing.
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Reviewed in the United States on March 25, 2026