SKU: 59165513275

Porcine IL-1β ELISA Kit

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Description

Porcine IL-1β ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich enzyme linked immunosorbent assay technology. Specific anti porcine IL 1 beta; The antibody was pre coated on a high affinity microplate. Add the standard, sample to be tested and biotinylated detection antibody into the wells of the enzyme plate. After incubation, the il 1&beta in the sample; Combine with solid phase antibody and detection antibody to form

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. Specific anti porcine IL-1-beta; The antibody was pre coated on a high affinity microplate. Add the standard, sample to be tested and biotinylated detection antibody into the wells of the enzyme plate. After incubation, the il-1&beta in the sample; Combine with solid-phase antibody and detection antibody to form immune complexes. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nm wavelength (refer to the correction wavelength of 540nm or 570nm).

Detection Type: double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Precoated 96 well plate, standard, anti porcine il-1β One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 3.8 pg/ml

Detection Range: 62.5 - 4000 pg/ml

Recovery Range: 69-102%

Storage Method: 2-8 ℃

Standard Curve

Background:

Interleukin 1 (IL-1)The protein family contains the canonical members il-1&alphaIl-1β And IL-1ra, as well as IL-18, IL-33, and il-1f5-10. Il-1α And il-1β It binds to the same cell surface receptors and shares biological functions. In addition to skin keratinocytes, some epithelial cells, and some cells of the central nervous system, unstimulated cells in healthy mice do not produce IL-1. However, as a response to inflammatory substances, infection or microbial endotoxin, macrophages and many other cells will produce IL-1 in large quantities. In the physiological process of immune response and inflammatory response, bone remodeling, fever, glucose metabolism, growth hormone / IGF-1, il-1β Plays a key role. Under a series of pathological conditions, including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myeloid leukemia, insulin-dependent diabetes mellitus, atherosclerosis, nerve injury and aging related diseases, IL-1 has been accompanied by abnormal or continuous production.

IL-1α And il-1β It has structurally related polypeptides with approximately 25% homology at the amino acid level. Both of them were synthesized as 31 kDa sized precursors and then cleaved into mature proteins with a molecular weight of approximately 17.5 kDa. Caspase-1 / ICeI cleaves il-1β Precursors are a key step in the inflammatory response. Il-1α And il-1β None of them contain typical hydrophobic signal peptides, but there is evidence that these factors can be secreted through non canonical pathways. A portion of unprocessed il-1α It can appear on the cell membrane and may have biological activity. With il-1α Different precursors, il-1β The precursors have little biological activity compared with the mature ones. Unprocessed and mature il-1β Can be exported from cells.

IL-1α And il-1β Functions through immunoglobulin superfamily receptors that bind to IL-1ra. Cells expressing 80kDa transmembrane type I receptor (IL-1 RI) include T cells, fibroblasts, keratinocytes, endothelial cells, synovial fluid inner layer cells, chondrocytes, and hepatocytes. Cells expressing 68kDa transmembrane type II receptor (IL-1 RII) include B cells, neutrophils, and bone marrow cells. The extracellular domains of these two IL-1 receptors share about 28% homology, but the significant difference is reflected in their intracellular domains: the intracellular domain of type II receptor has only 29 amino acids, while the intracellular domain of type I receptor has 217 amino acids. IL-1 RII does not produce signal for IL-1, and may act as a decoy receptor to weaken IL-1 function. IL-1 receptor accessory protein (IL-1 RACP) is associated with IL-1 RI and is required for IL-1RI signal transduction. IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1. Soluble IL-1RI and IL-1 RII can be detected in human plasma, synovial fluid and conditioned medium of various human cell lines. In addition, IL-1 binding protein similar to soluble IL-1 RII is able to be encoded by vaccinia and vaccinia virus.

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SKU: 59165513275

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c v
Dallas, US
★★★★★ 3
Not bad
Flavor Name: Superior Blend, Size: 1.4 Ounce (Pack of 1)
Great if you like a more subtle flavor, not bitter. If you just add it to milk then it has a slight very brief strong aftertaste, but I like mine a bit stronger. it mixed well it did not clump for me, it was a great vivid color. I will say I prefer a strong, bold matcha taste in my lattes so I do not think I would purchase again but overall it is a great option for beginners, people trying to figure out what they like, or folks who like a smoother, not bitter taste. The tin did come dented unfortunately, not a huge deal but also not great to receive a product that has been somewhat damaged.
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Reviewed in the United States on April 16, 2026
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Daryl
Boise, US
★★★★★ 5
Creamy, Smooth, and Fresh — Perfect First Matcha Experience
Flavor Name: Superior Blend, Size: 1.4 Ounce (Pack of 1), Flavor Name: Superior Blend, Size: 1.4 Ounce (Pack of 1)
I tried the Naoki Superior Blend Matcha for the first time and was honestly impressed from day one. I prepared it with milk, and the matcha turned out incredibly creamy and smooth with a natural sweetness — no bitterness at all. The color was a vibrant bright green, which immediately made it feel fresh and high quality. You can tell this is not one of those dull, stale matcha powders. It mixed well and had a very clean, balanced flavor. As someone who was looking for authentic Japanese matcha without the marketing gimmicks, this felt like a great find. Looking forward to trying it next with just warm water to experience the flavor on its own.
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Reviewed in the United States on May 26, 2026
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Charles
Carnegie, US
★★★★★ 5
Smooth like butter - Better than Jade leaf
Flavor Name: Superior Blend, Size: 3.5 Ounce (Pack of 1)
This stuff is smooth. No hint of bitterness in sight. Its so smooth I actually preferred to mix it 50/50 with Jade Leaf in my Matcha lattes. Its just amazing Matcha.. so good I started occasionally drinking it by itself not as a latte... the flavors present in just the tea (no milk, no sugar, etc) is insane. Umami, savory, very minimal mineral/bitter tastes. So if you like mineral tasting matcha, try Dens tea Restaurant Matcha... it has a very strong mineraly taste. This is almost the opposte. One thing to note, this stuff is so smooth and lacks bitterness that I actually have to use 3.5g instead of 3g like I do with Jade leaf... but once you get the strength dialed in it is MINT. I personally dont care about any clumping etc because I whisk my matcha with an electric frother in about 2 oz of water before adding milk or anything else.. even the matcha i had in my fridge which got clumpy from condensation didtn stand a chance... so if you ever have lumpy matcha.. its your fault, not the matchas fault. FYI. I dont mind the Jade leaf at all (for lattes)... in fact I wil gladly use it if its on sale.. but this is objectively the better matcha. If you plan to drink by itself, dont even consider jade leaf.. I am speaking specifically for lattes... Naoki is the only one you want to drink straight. But if you are a latte person.. either one is good quality and a buy again from me.
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Reviewed in the United States on March 20, 2026
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Freya
Lake Worth, US
★★★★★ 5
Best matcha hands down
Flavor Name: Superior Blend, Size: 1.4 Ounce (Pack of 1), Flavor Name: Superior Blend, Size: 1.4 Ounce (Pack of 1)
My favorite matcha I’ve gotten from Amazon thus far. I love the nutty flavor and it’s so creamy and the quality is 10/10 ! I highly recommend
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Reviewed in the United States on June 2, 2026
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Xiao Min Yuan
Los Angeles, US
★★★★★ 5
Great quality and value!
Flavor Name: Superior Blend, Size: 3.5 Ounce (Pack of 1), Flavor Name: Superior Blend, Size: 3.5 Ounce (Pack of 1)
After my Japan trip last year, I became a Matcha lover. Bought a few different brands, Naoki is the best and is my favorite. Taste fresh, smooth and love the bright green color. I drink Matcha latte everyday. Also whenever my friends come over, I would make one for them to show off my Matcha making skills. They love it as much as I do.
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Reviewed in the United States on March 26, 2026

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