Mouse CCL4/MIP-1β ELISA Kit

Detection Principle:
The double antibody sandwich ELISA method was used in this experiment. Anti mouse CCl4 monoclonal antibody is coated on the microplate. CCl4 in the sample and standard will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti mouse CCl4 multi antibody was added to combine with CCl4 bound on the microplate, and the free components were washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti mouse CCl4 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 8.3 pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85.0-108.9%

Storage Method: 2-8 ℃

Background:

CCL4 / MIP-1 beta is a β chemokine secreted by activated leukocytes, lymphocytes, vascular endothelial cells and lung smooth muscle cells at the site of inflammation. It attracts various immune cells to the site of microbial infection and other pathological inflammation, such as allergic asthma and ischemic myocardium.ccl4Is associated with CCL3 / mip-1α Heterodimers, secreted from activated monocytes. It signals through CCR5, and the N-terminally trimmed form also interacts with CCR1 and CCR2. In humans, the ability of CCl4 to bind CCR5 inhibits the cellular entry of m-directional HIV-1 utilizing CCR5 as a co receptor.