Pay in installments of $25.00 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 13 - Aug 18
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Cleaved PARP1 Recombinant Rabbit mAb (SDT-R084)Product Specification Host Rabbit Antigen Cleaved PARP1 Synonyms Poly [ADP ribose] polymerase 1, ARTD1, ADPRT 1, msPARP Immunogen N A Location Nucleus, Nucleolus Accession P11103 Clone Number SDT R084 Antibody Type Rabbit mAb Application WB, IHC P, IP, ICFCM Reactivity Hu, Ms Purification Protein A Concentration 0. 25 mg ml Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05 %BSA, 0. 03% Proclin 300 Stability & Storage 12 months from
Product Specification
| Host | Rabbit |
| Antigen | Cleaved PARP1 |
| Synonyms | Poly [ADP-ribose] polymerase 1, ARTD1, ADPRT 1, msPARP |
| Immunogen | N/A |
| Location | Nucleus, Nucleolus |
| Accession | P11103 |
| Clone Number | SDT-R084 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, IP, ICFCM |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05 %BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | |
| IHC-P | 1:1000 | |
| ICFCM | 1:250 | |
| IP | 1:25 |
Background
PARP1 possesses a nuclear localization sequence (NLS) near the DNA-binding domain and a caspase-cleavage site between the DNA-binding domain and the automodification domain [PMID: 10455009]. During caspasedependent apoptosis, PARP1 is cleaved by caspases 3 and 7 at its caspase-cleavage site into 24-kDa and 89-kDa fragments [PMID: 10497198, PMID: 8358726]. The 24-kDa PARP1 fragment contains the DNAbinding motif and the NLS, whereas the 89-kDa PARP1 fragment contains the automodification and catalytic domains. After PARP1 cleavage by caspase, the 24-kDa PARP1 fragment irreversibly binds to DNA breaks and acts as a transdominant inhibitor of active PARP1, whereas the 89-kDa PARP1 fragment is translocated to the cytoplasm [PMID: 11570811, PMID: 9721847]. Thereby, PARP1 fragmentation by caspase leads to its inactivation, which inhibits DNA repair and facilitates caspase-mediated DNA fragmentation in apoptosis.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy