SKU: 63100248136

Human IL-2 High Sensitivity ELISA Kit

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Description

Human IL-2 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 2 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 2 in the sample

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-2 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-2 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-2 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Somponents: A copy of pre coated 96 well plate, standard, IL-2 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.31pg/ml

Detection Range: 3.19-250 pg/ml

Recovery Range: 101-129%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 2 (IL-2) is a 15 kDa glycoprotein. In humans, it is encoded by a single gene located in the q26 – 28 region of chromosome 4. It is a class of signaling molecules in the immune system, regulating the immune function of leukocytes(white blood cells, usually lymphocytes) activity. IL-2 is the body's natural response to microbial infection, distinguishing foreign and self antigens. IL-2 functions by interacting with the IL-2 receptor, which is composed of three chains, namely α Chain (CD25), β Chain (CD122) and γ Chain (CD132). Monitoring the content of IL-2 in serum provides a more detailed perspective for a variety of pathological conditions, including cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

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SKU: 63100248136

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4.7 ★★★★★
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Stacie
West Palm Beach, US
★★★★★ 5
Great for large chewers
Color: Rubber Chew Toy, Size: Large
Our dogs very favorite toy. I have bought several in all colors. Good quality and it lasted a while even with my strong dogs.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 22, 2026
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cassie
Whiting, US
★★★★★ 5
Hard chewer pass!
Color: Rubber Chew Toy, Size: Large
Our dogs love these! They are hard chewers and am impressed they last a decent while. Good for the price!
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Reviewed in the United States on April 20, 2026
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Today
Dallas, US
★★★★★ 5
Airedale tough!
Color: Rubber Chew Toy, Size: Large
We have two young Airedale Terriers and I thought this would be a good inside toy for them to tug. And I was right! Very happily so. I have to admit I was surprised when I first saw it; it seemed so small. But the thing stretches and springs back to shape. We’ve had it about 2 weeks now and it is constantly played with. Absolutely the best toy! Very interactive either alone (it rolls) or with us or they play together. I am surprised how tough it is with all the constant rough play. Highly recommend!!!
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Reviewed in the United States on May 12, 2022
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Verified Purchase
Julie
Pawtucket, US
★★★★★ 4
Small, but twisty and fun looking. Seems relatively sturdy, though doesn't look indestructible at all.
Color: Rubber Chew Toy, Size: Large
SLIGHTLY disappointed because I thought this was bigger than it is. If you squish it all in close, it's maybe one and a half times the size of a tennis ball. (Of course, the size is probably listed; I think I just assumed, so my fault.) That being said, it's entertainingly twisty and squishy, though the rubber seems relatively sturdy. I'm not yet positive if it will hold up to the 8 month retriever/lab mix it's intended for; he tends to rip instantly through anything much less sturdy than kong natural rubber. But it seems like something that will amuse him, so we're going to give it a shot at Christmas.
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Reviewed in the United States on November 7, 2014
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Kindle Customer
Louisville, US
★★★★★ 5
Great product!
Color: Rubber Chew Toy, Size: Large
My dog is an aggressive chewer. I have bought this 3 times for her she loves to play tug and fetch with it. They last longer than most of the other toys I get her and it’s one of her favorites.
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Reviewed in the United States on January 1, 2025

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