Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 8 - Jul 13
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse NOG ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Noggin (NOG) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the Noggin (NOG) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Noggin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Noggin is a protein involved in the development of many body tissues, including nerves, muscle, and bone. Noggin is encoded by the NOG gene. The amino acid sequence of human Noggin is highly homologous to that of rat, mouse, and Xenopus (a genus of aquatic frog). Noggin is an inhibitor of several bone morphogenetic proteins (BMPs): at least BMP2, BMP4, BMP5, BMP6, BMP7, BMP13, and BMP14. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 103 reviews
Sort
Product Reviews
★★★★★ 5
Works great and easy to use
Kitchen gadgets have a way of accumulating. You buy them with good intentions, use them twice, and then they live in a drawer forever. This milk frother is not that. It's one of those rare small appliances that earns its spot on the counter every single morning.
The wireless charging is the feature that sets this apart from every other handheld frother on the market. No fumbling with a charging cable, no little dock that takes up extra space — just set it down and it charges. It sounds like a small thing until you've used it, and then it feels like the obvious way every handheld kitchen tool should work. The fact that it charges quickly means you're never waiting around or reaching for it only to find it dead.
Three speed settings give you real control over the result. Low for gentle mixing, high for a proper thick foam that holds up in a latte or cappuccino the way it should. The stainless steel whisk feels durable and substantial, not like something that's going to bend or corrode after a few months of daily use. The whole thing is well-made in a way you notice immediately, solid, balanced, and built to last.
The Starlight Black finish looks sharp, too. It's sleek enough to leave out without cluttering your kitchen aesthetic, which matters when you're using something every day.
If you make lattes or cappuccinos at home and you're still shaking a jar of milk or settling for flat foam, this is the upgrade you didn't know you needed. Easy to use, quick to charge, and genuinely well crafted.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
Three Speeds, Two Charge Options, Three Interchangeable Whisk Heads
I like this electric milk frother—it enables you to simulate café-style drinks at home. Three speed settings make it easy to switch from mixing matcha to whipping up thick, creamy foam for lattes. It also comes with three interchangeable whisk heads, so it is not just for coffee—I made French Toast with it the 1st time out. It can handle other tasks, like blending eggs, cream, and even protein shakes. The LED battery level display is up front right below the power button and shows state of charge as well as increasing battery level during the charging cycle. The dual charging options—either the magnetic charge stand or the USB-C charge port on the side of the motor—make it convenient for home or for travel. Best of all, all the detachable whisk heads clean up fast, so using it daily does not feel like a chore. I will say that I prefer a momentary one-speed switch instead of cycling the speeds (9K RPM, 11K RPM & 13K RPM) with the power switch. It spins up very fast and to shut it off you have to keep pressing the power button through faster speeds and before you can shut it off, it overflows the cup sometimes. You can also long press the power button to turn it off, but it usually takes too long and still overflows—I wish it was more controllable. I found that the corkscrew-hook head is the best of the three to tame that effect.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 26, 2026
★★★★★ 4
Good companion to a coffee setup
This electric frother is a great companion to have in a kitchen/coffee setup and I've been using it to mix my morning coffee.
The frother includes a stand which serves as the charging base and multiple attachments (likely won't really see too much use realistically). My only downside is that the stand situation is a bit lackluster as the wireless charging feature requires you to line up the two pins at the top to the stand and balance the frother. Alternatively the frother also has a USB C charging port (sadly not USB C compliant as it can't be charged via USB c to c).
The frother itself cycles between different levels of speed when you press the button and it is pretty strong; being able to very easily swirl and mix large amounts of coffee leaving mine nice and frothy. I've also been using this to mix drink powder mixes to great effectiveness.
The other attatchments don't really hold too much value to me (other than maybe the whisk for eggs) but is nice to have other options included. Overall this frother is a massive step up over me using a spoon to stir my coffee.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
★★★★★ 5
My coffee is now judging your coffee.
I’m a simple man. All I wanted was decent foam on my morning coffee without paying $7 at Starbucks like a sucker. What I got instead is this charcoal black, LED-equipped, triple-whisk, rocket-powered milk frother that feels like it should have a NASA logo on it.
This thing has three speeds and an actual digital battery display. I caught myself staring at the 1% accuracy battery percentage like it was the stock market. “We’re at 87%… we’re still frothing kings today.” The magnetic charging dock is so satisfying it should be illegal. I literally say “goodnight” to it when I put it back on the stand.
The spring whisk? Velvety microfoam so perfect my wife asked if I was having an affair with a barista. The balloon whisk made whipped cream so fluffy my kids thought it was a cloud that fell into their hot chocolate. And the hook whisk? Blended my protein shake with zero lumps, a miracle previously only achieved by professional athletes and people with patience.
Battery life is ridiculous. I’ve been using it twice a day for two weeks and it’s still at 64%. This frother could survive a zombie apocalypse better than I could.
The only real downside? I now judge other people’s coffee foam in public. Saw a sad flat latte the other day and whispered “that man doesn’t own the Dropology frother…”
If you like coffee, matcha, hot chocolate, or just feeling like a kitchen god, buy this immediately. It’s the most over-engineered $30-ish gadget I’ve ever loved.
10/10 would froth again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 22, 2026
★★★★★ 5
Powerful and I love the charging base and attachments
This is surprisingly more powerful than I thought it would be. It will whip up an egg for scrambled eggs as well as froth your milk. It came with 3 attachments and a charging base. You can charge it using the base or directly in to the frother using the included usbc. I have had battery operated frothers that really weren't that great. This one is blows them all away. I use it mainly to stir my coffee and scramble my eggs and I'm sure I will find many more ways to use it. Its a great value and looks nice on my counter top.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026