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Description
Human Fascin, His tagProduct Specification Species Human Synonyms 55 kDa actin bundling protein, Singed like protein, p55, FAN1, HSN, SNL Accession Q16658 Amino Acid Sequence Protein sequence(Q16658, Met1 Tyr493, with C 10*His)
Product Specification
| Species | Human |
| Synonyms | 55 kDa actin-bundling protein, Singed-like protein, p55, FAN1, HSN, SNL |
| Accession | Q16658 |
| Amino Acid Sequence | Protein sequence(Q16658, Met1-Tyr493, with C-10*His) |
| Expression System | E.coli |
| Molecular Weight | Theoretical: 56.2kDa Actual: 56kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
| Reconstitution | Reconstitute no more than 0.1 mg/mL according to the size in deionized water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, -20 to -70 °C as supplied. |
Background
Fascin is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin (from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer, pancreatic cancer, and prostate cancer. It is also helpful in identifying Hodgkin cells.
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