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Description
FLT-3L Protein, MouseProduct Specification Species Mouse Synonyms FL; FLG3L; Flt3 ligand; Flt 3 Ligand; Flt3L; FLT3LG; fms related tyrosine kinase 3 ligand; SL cytokine Accession P49772 Amino Acid Sequence Gly27 Arg188, with C terminal His Tag Expression System HEK293 Molecular Weight 20 33kDa(Reducing) Purity 95% by SDS PAGE Endotoxin <0. 1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4. Reconstitution
Product Specification
| Species | Mouse |
| Synonyms | FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine |
| Accession | P49772 |
| Amino Acid Sequence | Gly27-Arg188, with C-terminal His Tag |
| Expression System | HEK293 |
| Molecular Weight | 20-33kDa(Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1. Cancer Res. 2009 Oct 1,69(19):7747-55. 2. Blood, Jul 2009, 114: 835 - 843.
3. J. Immunol., Jun 2009, 182: 7408 - 7414.
|
Background
FLT-3 ligand promotes long- term expansion and
differentiation of human pro-B-cells in the presence of IL-7 or in combination
of IL-7 and IL-3.The human FLT-3 ligand also stimulates the proliferation of
cells expressing murine FLT-3 receptors. In combination with SCF and IL-3,
FLT-3 ligand can cause expansion of cells with the marker spectrum CD34(+)
CD38(-). Alone, FLT-3 ligand supports the survival of precursors in the lineage
of blood-forming cells such as CFU-GM, CFU- GEMM, and the very primitive high
proliferative potential colony-forming cells, HPP- CFC. flt-3 ligand only has
marginal effects on progenitors for erythroid cells and megakaryocytes.
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