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Description
Unione ®️ TR-FRET Human PD1/PD-L1 Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit utilizes homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between PD1 and PD L1. This method enables simple, rapid, and high throughput screening of inhibitors and antibody blockers. As shown in the figure, the interaction between PD1 and PD L1 is detected using an Eu labeled anti Tag1 antibody (TR FRET donor) and an Ac labeled anti
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
The assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.
As shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.
Components
Component |
Concentration |
100T |
500T |
2500T |
10000T |
Storage Temperature |
Pembrolizumab |
5μM |
5μL |
10μL |
50μL |
200μL |
-80℃ |
Tag1-PD-L1 protein |
100× |
5μL |
20μL |
100μL |
400μL |
-80℃ |
Tag2- PD1 protein |
100× |
5μL |
20μL |
100μL |
400μL |
-80℃ |
Anti-Tag1 Eu antibody |
50× |
10μL |
50μL |
250μL |
1000μL |
-80℃ |
Anti-Tag2 Ac antibody |
12.5× |
40μL |
200μL |
1000μL |
4000μL |
-80℃ |
Detection buffer |
10× |
400μL |
2mL |
10mL |
40mL |
-80℃ |
Note: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy