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Description
Annexin V-APC/7-AAD apoptosis analysis kitProduct Specification Usage 1. Instrument parameter adjustment 1. Collect 1 106 3106The cells were washed twice by centrifugation with pre cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre cooled 1 Binding Buffer to resuspend, and add the same
Product Specification
| Usage | 1. Instrument parameter adjustment 1. Collect 1 × 106-3×106The cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre-cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes. 5. Add 5 uL of Annexin V-APC or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light. 6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube Compensation of channels. Note:Certain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase. 2. Sample testing 1. Induce apoptosis according to the experimental protocol. 2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells (including cells in the culture supernatant). Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells. 3. Add 5 uL of Annexin V-APC and 10 uL of 7-AAD per tube. 4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes. 5. Get on the computer for analysis. |
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| Beilstein | 0 | |||||||||||||||
| Synonym | Annexin V-APC/7-AAD Apoptosis Analysis Kit | |||||||||||||||
| Description |
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V Kit Components:
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| PubChem CID | 0 | |||||||||||||||
| General Notes | 1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic. 2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment. |
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| Storage Temp. | Store at 2-8 °C, do not freeze, shelf life 1 year. |
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4.6 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Fun Ball
Style: Ball, Size: Medium (Pack of 2)
Great new ball for our pup. He loves the sound of the crunch!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
★★★★★ 5
Durability
Style: Ball, Size: Medium (Pack of 2)
My dog loves these balls!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
★★★★★ 5
Dogs love them
Style: Ball, Size: Medium (Pack of 2)
Our dogs love these. What’s great is that you can add more plastic (like from water bottles) if the plastic starts to fall out from the center. If you have a chewer, though, these won’t last you long. Our shepherd mix loves these and then starts to pull the rubber apart the more she plays with it. Our other dogs treat it normally, though, and those ones last forever. They also get some decent height when bounced, and they make a unique whistling noise when thrown.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 17, 2025
★★★★★ 4
Our pup loves the cronch cronch!
Style: Ball, Size: Medium (Pack of 1)
I bought our 15 month old Coton several Chuck-It toys on an Amazon deal. He is incredibly playful, mischievous, and ball/toy obsessed, especially for anything that makes noise. He can also be very destructive so I love how durable most of their products are - they’re among our longest lasting toys and worth every penny. We have yet to take them to the dog park, but they’re a perfect size for sharing in play time. And they are keeping him busy at home - that’s a good thing.
He loves investigating the crunchy-cronchy-crinkly sound which is a nice change from squeakers. It can withstand his destructive chewing well-being, gentle on his little teeth. I love that. Chuck-It is thinking outside the box with a variety of balls to keep dogs engaged! I do worry about the crinkle plastic inside because it is accessible because of the air holes and it feels a little sharp. So this will have to be an at home toy so we can monitor his play.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Great products all around
Style: Ball, Size: Medium (Pack of 2)
Any of these balls are great! My dogs love all of them. The squeaky ones are their favorite!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 12, 2026
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