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Description
Alexa Fluor® 647 Rat Anti-Mouse CD144 Antibody (S-R719)Product Specification Host Rat Antigen CD144 Synonyms Cadherin 5; Vascular endothelial cadherin (VE cadherin); Cdh5 Location Cytoplasm, Cell membrane Accession P55284 Clone Number S R719 Antibody Type Rat mAb Isotype IgG1,k Application FCM Reactivity Rt Positive Sample bEnd. 3 Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12
Product Specification
| Host | Rat |
| Antigen | CD144 |
| Synonyms | Cadherin-5; Vascular endothelial cadherin (VE-cadherin); Cdh5 |
| Location | Cytoplasm, Cell membrane |
| Accession | P55284 |
| Clone Number | S-R719 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Rt |
| Positive Sample | bEnd.3 |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Ms |
Background
CD144, also known as VE-cadherin or cadherin-5, is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail, expressed almost exclusively at the adherens junctions of endothelial cells in blood and lymphatic vessels, where it mediates homophilic adhesion, controls endothelial permeability, maintains vascular integrity, promotes endothelial quiescence and tubulogenesis, and is indispensable for embryonic vascular development since its genetic deletion or truncation causes mid-gestational lethality due to vessel disassembly and hemorrhage.
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