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Description
Rat SIRT7 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with NAD-dependent deacetylase sirtuin 7 (SIRT7) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of NAD-dependent deacetylase sirtuin 7 (SIRT7) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat NAD-dependent deacetylase sirtuin 7 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Sirtuin-7 (SIRT7), also known as sirtuin 7 or NAD-dependent deacetylase sirtuin 7, is an enzyme encoded by the SIRT7 gene. It is a member of the sirtuin protein family and is homologous to the yeast Sir2 protein. It promotes transcription by DNA polymerases I, II, and III. It is expressed more in metabolically active tissues, such as the liver and spleen, and less in non-proliferating tissues, such as the heart and brain. It is essential for rDNA transcription. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.3 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 4
Not like the pictures but well worth the money
Size: Queen, Style: 1 Green
4 stars because it is not nearly as padded as the descriptions and images make it seem. The filling flattens easily and doesn’t provide any support.
That being said it does make my bed more comfortable and soft and has improved my sleep quality. I have a 12 in soft/medium firm memory foam mattress that was wonderful for the last +2 years till my back surgery ~6 months ago. Since then my joints ached when trying to sleep and when waking, my back would be all out of place and achey, and I was untested. Since buying this topper these issues have improved by 80% or more. It is a great addition to my bed and I don’t regret buying it.
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Reviewed in the United States on May 18, 2026
★★★★★ 5
Pleasantly surprised!
Size: Queen, Style: 2 Dark Gray
I had been searching for a quality mattress topper in hopes of extending the life of our mattress. I compared a few options and kept coming back to this one. I have to admit I was skeptical because the price point was lower than many options. This topper is SO comfortable and soft! I left it laying out for a couple hours and then followed the instructions to put it in the dryer. Worked like a charm. I’ve been going through issues with hip pain and this has made a difference in my sleep and I’m waking up in less pain. So happy I tried this out. The padding is so comfortable and fit on the mattress using the same sheet we had.
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Reviewed in the United States on May 17, 2026
★★★★★ 5
So perfectly soft
Size: King, Style: 2 Dark Gray
My husband was complaining about the mattress, and wants to spend a lot of money on a new one like the ones at the Waldorf. I asked him to give me a chance to try to fix it first. Watched youtube videos about hotel style beds, and landed on the need for a mattress topper.
This one is so... very... comfortable. I thought the grid pattern would bother me, but nope. You don't realize how much heat you soak up from your mattress until you get a nice airy layer in between. I almost completely stopped sweating at night, and he's stopped complaining about hurting as much.
This is another of those items that I'm scared of washing too much, but it's held up with care and caution. If it starts to get a bit too squished, I just fluff it in the dryer and the padding poofs right back up. The sides are deep and my mattress sinks right into the pocket with no pulling. I have bad back pain and I am certain this has been helping support my back a bit better, so I wake up more refreshed. I'm sorta popping out of bed like I used to years ago.
He still plans on a new mattress, but this has saved us money, for now, and when we get the new one, this will layer on top like a dream. I'm glad I took the chance on this, it has really improved our rest, and that's so important.
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Reviewed in the United States on May 16, 2026
★★★★★ 5
Cloud like plushness without loss of support
Size: Queen, Style: 1 Green
Cloud like plushness while not losing support. Bought a new high end plush mattress and adjustable bed frame with massage and still wasn’t feeling that cloud like feeling that I like. I don’t like foam toppers because it is too soft and it lacks support. This topper fit perfectly on a 13” tight top mattress over the protector. The topper was exactly what I was looking for. So comfortable, cool, and put me to sleep right away. Massage feature on adjustable base feels the same as it did without the topper, but the bed is just more comfortable and hard to not want to be lazy in it all day now. For the price, this can’t be beat. If you’re looking for a mattress topper to enhance the comfort of what you already have, buy this. You won’t be disappointed!
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Very, very soft and cushiony that I can feel even thru the fitted sheet .
Size: King, Style: 1 Green
Very well packaged. Box was very heavy, but once I got thr shrink wrapped package opened, it inflated on its own. It is a perfect fit. Although it isn't thick, it is extremely soft and my back and hip feel better (arthritis and sciatica). I plan on getting a memory foam pad to put underneath, but for now, I am reasonably pleased. The quality was so good, I decided to keep it instead of going thru the trouble of repacking it.
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Reviewed in the United States on May 25, 2026