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Description
Mouse IL-2 ELISA KitProduct Specification Theory Double antibody sandwich enzyme linked immunosorbent assay was used in this kit. Specific anti mouse IL 2 antibody was precoated on a high affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL 2 present in the sample combined with the solid phase antibody and detection antibody to form immune complexes. After washing to
Product Specification
| Theory | Double antibody sandwich enzyme-linked immunosorbent assay was used in this kit. Specific anti-mouse IL-2 antibody was precoated on a high-affinity microplate. The standard substance, test sample and biotinylated detection antibody were added into the microplate Wells. After incubation, the IL-2 present in the sample combined with the solid-phase antibody and detection antibody to form immune complexes. After washing to remove the unbound material, the horseradish peroxidase-labeled Streptavidin-HRP was added. After washing, the chromogenic substrate was added and the color was developed in the dark. The reaction was terminated by adding the termination solution, and the absorbance value was measured at 450nm wavelength (reference correction wavelength 540nm or 570nm). | |||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-2 (IL-2, also known as TCGF) is a 15-16 kda monomeric α-helical glycoprotein belonging to the type I cytokine family. Mouse IL-2 precursor is a 169-amino acid (AA) peptide containing a 20-amino acid signal sequence and a 149-amino acid mature chain. There is an additional potential site for O-linked glycosylation, as well as a polyglutamine region at residues 35 to 46. It is worth noting that mouse IL-2 has multiple alleles, resulting in differences in the number of glutamine at amino acids 21-46 and the number of TSSS repeats in the mouse IL-2 sequence (SwissProt#:P04351). The amino acid sequence of mature mouse IL-2 shared 56% and 73% identity with human and rat IL-2, respectively, with the major difference located in the polyglutamine region. There is some cross-reactivity between mouse and human IL-2. Functionally, IL-2 was originally recognized as a T-cell growth factor and, therefore, was used in the treatment of cancer and AIDS patients, as well as to enhance the immune response. However, these efforts have had limited effect. "Experimental studies have shown that mice lacking IL-2 or its receptor also develop lymphoproliferation and autoimmune diseases, suggesting that IL-2 is a growth-limiting rather than growth-inducing factor." Recent studies have shown that the real cause of lymphocyte proliferation is the inability to produce CD4+FoxP3+CD25/IL-2Rα+Treg cells. It is important to note that now IL - 2 is considered successful induction of CD8 + memory cells necessary for memory response. It is well known that mammalian cells expressing IL-2 include CD4+ and CD8+T cells, NK cells, NKT cells, and dendritic cells. The IL-2 receptor is complex and consists of three different subunits. The two subunits can be combined with ligands, called IL - 2 r alpha and beta IL - 2 r. IL-2Rα is 55kDa and binds IL-2 with low affinity. IL - 2 beta for 75 kda, is part of the IL - 15 receptors, moderate affinity with IL - 2. Signal transduction is mediated by IL-2Rβ and a 64kDa common γ chain (γc). Gamma c and IL - 4, 7, 9, 15-21 and Shared receptors. Signal transducerγc does not bind to IL-2 but heterodimerizes with IL-2Rβ to form a functional IL-2 receptor. Heterologous trimer alpha beta gamma receptor may be combined with IL - 2 c, forming R alpha beta complex R. | |||||||||||||||||||||||||||
| General Notes | 1. Please use the kit within the validity period. 2. Components of different kits and different batch kits should not be mixed. 3. If the sample value is greater than the highest value of the standard curve, the sample should be diluted with (1×) The samples were diluted and retested. If the cell culture supernatant sample needs to be diluted, except for the last step, diluent (1×) is used. "In addition to dilution, cell culture medium may be used for other intermediate dilutions." 4. The difference of the test results can be caused by a variety of factors, including the operation of the experimenter, the use of the pipettor, the washing technique, the reaction time or temperature, the storage of the kit, etc. 5. The termination solution in the kit is acidic. Please protect your glasses, hands, face and clothes when using it. 6. For scientific research only, not for in vitro diagnosis. |
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| Storage Temp. | Unopened kit, 2-8° C Storage | |||||||||||||||||||||||||||
| Test Range | 15.6pg/mL-1000pg/mL |
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4.1 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 4
Rechargeable reliable
Color: Lux Alpha Black, Size: Rechargeable Z1 Motor Max
Nice, very reliable and easy-to-use and clean
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 20, 2026
★★★★★ 5
Great look and very powerful - consider what your wanting to froth/mix - may be too powerful
Color: Lux White, Size: Rechargeable Z1 Motor Max
It's a great frother - but more for large drinks. Very powerful, even on the low speed. I returned it because I want something to mix my collagen and creamer in my coffee. The first time I turned it on it shot half the coffee out of the cup - very powerful. It has a great look, sleek and fits easy in the hand - but for what I needed, two powerful. It would have been nice to have a fully adjustable speed wand like my replaceable battery powered frother.
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Reviewed in the United States on April 1, 2026
★★★★★ 5
Powerful, rechargeable battery, easy to use & clean
Color: Lux Absolute Silver, Size: Rechargeable Z1 Motor Max
2 speeds, easy to change back and forth to different speeds with one click , easily blends 1/4 cup protein powder in 12 oz milk on low speed. Easy to clean: Stick blending wand in tall glass with warm water + dish soap, turn on low speed to clean easily (don’t stick the part that holds the battery in glass). Also of note, chosen by Americas Test Kitchen as best milk frother but I use it to make protein shakes.
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Reviewed in the United States on June 5, 2026
★★★★★ 5
Works great on bullet coffee!!
Color: Lux Alpha Black, Size: Rechargeable Z1 Motor Max
I am really pleased with this frother! Frothers I have used in the past didn’t mix my decaf coffee, 2% milk, and MCT oil as thoroughly as I like (oil and coffee separated). This frother blends everything AND I get a nice layer of frothy milk on top!!! Charge lasts a long and the froth can be submerged all the way up to the button!!!
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Reviewed in the United States on June 5, 2026
★★★★★ 5
Perfect for Lattes
Size: 20 Ounces
Good for steaming 8-10 oz of milk. The nifty tool is good for scooping out your puck.
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Reviewed in the United States on February 19, 2026
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