SKU: 68082240984

Human IFN-γ OneStep OptiQuant ELISA Kit

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Description

Human IFN-γ OneStep OptiQuant ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 2 to 8 C as supplied. Kit Precision Intra assay: 4. 0% Inter assay: 4. 5% Sample type Cell culture supernatant, Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 2. 577pg ml Range 15. 63 pg mL 1000 pg mL Recovery Cell culture supernatant: 93% Serum: 109% Plasma: 111% Assay time 45 minutes

Product Specification


Antigen IFN-γ
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage

2 to 8 °C as supplied.

Kit


Precision Intra-assay: 4.0%;
Inter-assay: 4.5%
Sample type Cell culture supernatant, Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 2.577pg/ml
Range 15.63 pg/mL – 1000 pg/mL
Recovery Cell culture supernatant: 93%
Serum: 109%
Plasma: 111%
Assay time 45 minutes
Species reactivity Human
NIBSC convert

The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).

Background

Interferon-gamma (IFN-γ) is a dimeric cytokine central to innate and adaptive immunity, primarily produced by activated T cells and natural killer (NK) cells. Structurally, IFN-γ exists as a homodimer, with each subunit comprising six α-helical domains stabilized by intra- and interchain disulfide bonds. It signals through a heterodimeric receptor complex composed of IFNGR1 and IFNGR2, initiating JAK-STAT pathway activation. Post-translational glycosylation at asparagine residues (e.g., Asn25 and Asn97 in humans) enhances its stability and modulates receptor interaction dynamics. Immune Regulatory Roles and Therapeutic Cross-Talk of IFN-γ: Interferon-gamma (IFN-γ) orchestrates multifaceted immune responses, exerting critical antiviral and antitumor defense by inducing cellular antiviral states and enhancing tumor immunosurveillance through upregulation of MHC class I/II expression. It activates macrophages to bolster phagocytosis, reactive oxygen species production, and pathogen clearance, while polarizing CD4+ T cells into Th1 subsets to amplify cell-mediated immunity. Despite its protective roles, IFN-γ exhibits a dual nature in inflammation, where excessive levels contribute to autoimmune disorders (e.g., rheumatoid arthritis, multiple sclerosis) and chronic inflammatory pathologies. Therapeutically, IFN-γ synergizes with proinflammatory cytokines like TNF-α and IL-12 to amplify immune activation, while counteracting the immunosuppressive effects of IL-4 and IL-10, highlighting its pivotal yet complex role in balancing immune defense and inflammatory regulation. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IFN-γ quantification in time-sensitive or resource-limited settings.

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SKU: 68082240984

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Amazon Customer
Omaha, US
★★★★★ 4
Four Stars
Format: Paperback
It is what I wanted
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Reviewed in the United States on March 19, 2018
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Kelly Kerr
Alexandria, US
★★★★★ 5
Brilliant! I have been convinced for that the opening ...
Format: Paperback
Brilliant! I have been convinced for some time that the opening verses of I John were speaking about the resurrection and not the incarnation per se. Not that these two are opposed to one another, which is evident from Jensen's title: resurrection presupposes incarnation, but what John has specifically in mind in his letter's opening is the resurrection. So, when John says in his opening "That which was from the beginning, which we heard, which we have seen with our eyes, which we have looked upon, and have touched with our hands concerning the word of life..." What was heard, seen, looked upon, and touched was the resurrected Christ (cf. John 20:24-29). And next, "the life was manifest" would mean the new life Jesus was resurrected to, that is eternal life never to die again. And now what John has seen and heard concerning the resurrection he has proclaimed to the recipients of his letter. Moreover, it is the resurrection that confirms that Jesus is the Christ; it is his vindication by the Father that he was exactly who he claimed to be and was unjustly put to death by his fellow Jews. This then means that the major conflict taken up within the letter is over the resurrection. To deny the resurrection is to deny that Jesus is the Christ and vice versa. In fact, this is the spirit of the antichrist. Jensen goes into much more detail than this, and provides a reading of the entirety of the letter through the lens of the resurrection. His reading is overall successful and convincing. There is a portion here or there that I would differ from him, but Jensen's thesis is sound and makes remarkable sense of John's epistle. I highly recommend this book.
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Reviewed in the United States on July 27, 2017
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Cookie
Louisville, US
★★★★★ 5
LOVE MY SINK CADDY!
Color: Black
This caddy is such a game changer for my kitchen. It keeps everything neat and in one place AND no more clutter around the sink! I can fit my soap, sponges, and brushes easily, and it still looks nice on the counter. The drying base really works too! It absorbs water fast and doesn’t get gross like other trays I’ve had. It’s sturdy, doesn’t slip, and even looks kind of decorative. Definitely worth it!
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Reviewed in the United States on October 28, 2025
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jnoc
Belleville, US
★★★★★ 5
Great product.
Color: Black
I love it. Good design , well made and very large capacity. Easy to assemble. Keeps my counter free from cleaning items and drys out my sponge so it doesn’t get mildew. It’s very stable , not flimsy at all. Great price too.
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Reviewed in the United States on April 1, 2026
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Amy C.
Lake Worth, US
★★★★★ 5
Great Sink Caddy
Color: Black, Color: Black
Great size and you can configure it to accommodate a range of soaps and gadgets. The brush holder can be moved to the outside for more storage area in the main basket. The little divider adjust across the wires and it stays in place. The stone bottom absorbs drips quickly and I haven’t noticed any discoloration on it. It stays in place on the sink edge. Perfect for my organization needs and keeps the area neat vs tossing the brushes on the ledge leaving water marks on the counter. It was packaged well and the coating seems good! I bought a cheaper one and it came bent, with the coating popped off in several places. This one is worth paying a little bit more for the quality!
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Reviewed in the United States on April 7, 2026

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