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Description
mIHC Dye (TSA540)Description mIHC Dye (TSA540) is a high performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin fixed paraffin embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi target, in situ biomarker detection in a single tissue section. This reagent provides strong, stable
Description
mIHC Dye (TSA540) is a high-performance fluorescent dye designed for multiplex immunohistochemistry (mIHC) based on tyramide signal amplification (TSA) technology. It enables robust, covalent fluorescent labeling on formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections, and tissue microarray (TMA) slides, supporting multi-target, in situ biomarker detection in a single tissue section.
This reagent provides strong, stable fluorescence with Ex/Em: 520 nm / 540 nm, compatible with standard fluorescence microscopes and multispectral imaging systems. It delivers greatly amplified signals compared to conventional immunofluorescence, ideal for low-abundance antigens and spatial profiling of the tissue microenvironment.
Key Features
TSA-based signal amplification: Ensures high sensitivity and high signal-to-noise ratio, suitable for detecting low-abundance antigens.
Covalent binding: Binds to tyrosine residues covalently, maintaining excellent stability during antibody stripping and repeated staining cycles.
Broad Compatibility: Applicable to FFPE tissues, frozen sections, and TMA slides, meeting diverse experimental needs.
Spectral Characteristics: 540 nm spectral channel, matching AF514, Opal 540, and similar orange-yellow fluorophores.
Easy to Use: Ready-to-use formulation with included signal amplification reaction buffer, no complex pre-processing required.
Application Scope: For research use only; not for in vitro clinical diagnosis or human trials.
Principle
TSA540 adopts horseradish peroxidase (HRP)-conjugated secondary antibodies to activate fluorescently labeled tyramide substrates. The activated tyramide rapidly forms covalent bonds with tyrosine residues near the antigen-antibody complex, forming stable fluorescent deposits. After that, the antibodies can be stripped by heat repair without removing the dye signal, allowing sequential labeling of multiple targets on the same tissue section to achieve multiplex staining.
Storage & Stability
Storage Condition: Store protected from light at 2–8 °C; avoid direct exposure to strong light.
Shelf Life: Stable for 12 months from the date of receipt when stored according to the required conditions.
Composition
TSA Monochromatic Fluorescent Dye 540, Signal Amplification Reaction Buffer
Application
Mainly used for scientific research of immunohistochemical staining of tissues, paraffin sections, and TMA chips; not for in vitro clinical diagnosis or human trials.
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4.4 ★★★★★
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Product Reviews
★★★★★ 5
Stylish way to stay organized!
Color: Black
This tray is a perfect way to keep your everyday items organized and easy to find when you are getting ready for your day. No more wondering where you've put your keys, your glasses, your wallet, your watch or your every day wear jewelry like your wedding ring (if you have to remove it at night due to hands swelling or similar). This tray allows my husband to be ready to go quickly if we decide to spontaneously go somewhere. Its compact enough to fit on a night stand or a dresser, but still provides adequate spaces for your every day pocket items like a multi-tool or pocket knife, spare change, etc. Its well made and stylish too. I am very pleased with this purchase.
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Reviewed in the United States on December 1, 2025
★★★★★ 5
Great and good looking storage
Color: Black
Very nice quality, I like the material a lot.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
Nice night stand tray!
Color: Brown
Great material and size! Holds all the trinkets that are kept on the night-stand. Helps to keep things nice, neat, and organized.
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Reviewed in the United States on January 19, 2026
★★★★★ 5
Perfect for my boyfriend!
Color: Black
My boyfriend has been wanting an organizer for all of his man things, and this was the perfect one! And super affordable! It’s very sleek and looks great on his night stand!
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Reviewed in the United States on January 6, 2026
★★★★★ 4
Great gift
Color: Black
Functional and good for organizing
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Reviewed in the United States on April 6, 2025