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Description
Human C4BPb ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 2000 pg/mL). Then dilute to the following concentrations: 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 2000 pg/mL standard working solution into the first EP tube and mix thoroughly to make a 1000 pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against C4 Binding Protein Beta (C4BPb). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of C4 Binding Protein Beta (C4BPb) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human C4 Binding Protein Beta ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | C4 binding protein β (C4BP) is a protein complex involved in the complement system, where it plays an inhibitory role. C4BP has an octopus-like structure with a central stalk and seven branched α-chains. The predominant form of C4BP in human blood is composed of seven identical α-chains and a unique β-chain, which in turn binds to the anticoagulant, vitamin K-dependent protein S. C4BP is a large glycoprotein (500 kDa) with an estimated plasma concentration of 200 μg/mL and is primarily synthesized in the liver. The genes encoding the C4BP α-chain (C4BPPA) and β-chain (C4BPB) are located in the regulator of complement activation (RCA) gene group on the long arm of chromosome 1, adjacent to other complement inhibitors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 31.2-2,000pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.6 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 5
Comfortable and useful
Size: Full Size, Pattern Name: Keyboard
Amazing support for all day typing.
Comfortable, great length!
Functional, easy cleaning if needed!
Great value for money. Stays in place.
Just what I needed for my time in the office.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 26, 2026
★★★★★ 5
The best wrist rest I've used.
Size: Compact, Pattern Name: Keyboard
It has just the right balance of firmness and softness. The cooling gel does keep it cool. It is durable. Its rubber grip holds it firmly on my desktop. If I need another one, I will purchase this product.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 5
It's fantastic. It's just not quite perfect.
Color: Black, Size: Full Size
Update after almost 2 years of ownership:
I've gotten very used the the height of the full height keyboard wrist rest. I can't go back to a smaller one. It's super comfortable. I bought a second one for work and 2 mouse wrist rests to go along. All of them are built fantastically. They clean easily. If something happened to one of the ones I already have, I would come straight back here to buy another of the same one. On point #2 in my original review below, I still wish the edges weren't tapered so much, but I have gotten used to it. I gave in and offset the wrist rest from the keyboard. Not ideal, but it gets the job done.
Bottom line: would recommend any time.
Original review:
The build quality is good. The fabric is nice and soft. The bottom keeps it from sliding. It seems great, overall. I have only 2 problems with this thing:
1. It's too thick. I should have gone with the thinner one. Maybe it'll wear out a bit and my hands will lower into a position with which I'm more comfortable.
2. The left and right sides taper off. It's fine that the front and back do, but having the sides tapered means that if I center this along the bottom of my keyboard and rest my hands to type, my left hand is partially sloping off to the left side, off of the rest. Obviously this is easily fixed by sliding the rest to the left of center, but I've got a compulsion about things lining up and this really really digs into my brain badly.
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Reviewed in the United States on April 4, 2017
★★★★★ 5
By about last year I started looking at more pricey wristpads like the leather wrapped ducky and several other imitations online
Color: Black, Size: Full Size, Color: Black, Size: Full Size
I've been using Grifiti wristpads for a few years now at home and work. I buy a replacement every 6/9 months due to them getting absolutely filthy and having the top fabric falling apart. By about last year I started looking at more pricey wristpads like the leather wrapped ducky and several other imitations online. I decided to wait and at one point heard of Glorious although I was on a new set of Grifiti's so didn't think a lot of it.
So the time came for new pads (once again!) and when I was checking for alternatives to the Grifiti (and the Leather from EliteKeyboards, Ducky, Filco, and other wristpads -- sheesh, I'd think I was talking keyboards there with names). Then I came across the Glorious and recalled its a higher quality wristpad so I decided to order a full and compact to use at work. Definitely very happy with them so far and by 6 months and a year then I'll see how they hold out.
Pros:
* Cushion-y soft great for resting and supporting wrists, hands, or an arm on
* Firm without being too hard or soft
* Cloth wrapped so they're not going to wear apart like a Grifiti
* Cloth is sewed to the base and not glued by inspection
* Doesn't slide, there's a textured non-slick rubber on the bottom. Great on my work desk!
* Good price, the leather imitation glued to a base starts at $30+ dollars and they go up to $70
Cons:
* None so far!
I'll be buying 1 more for home use in a month as im using both full and compact at work. The compact size matches up well with my mouse pad there although you may want to measure if you do this. May Gaben be with you!
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Reviewed in the United States on August 18, 2015
★★★★★ 4
Overall great
Color: Black, Size: Mouse Wrist Pad
All positive things to say except one mildly negative comment which is also personal preference.
Positives
1. Made well, doesn't fall apart or tear at the seams.
2. Perfect size for a mouse wrist rest
3. Comfortable for average duration usage, 2-5 hours
4. Simple yet elegant design, nothing over the top or "gamery" (It is a freakin wrist rest folks, it looks good and works)
5. Non slip rubber does keep it from sliding around the majority of times on the majority of surfaces such as glass and metal however on a slick laminate I find it still slides about a bit. It works well but when they look to the future perhaps an eval on this would help.
Negatives (Take with a grain of salt as I suffer from both Arthritis and Carpal Tunnel from 20 years of long duration PC use)
1. Too firm and not contoured to cradle your wrist. Because of this I find for my use (12-16 hours daily as I work and play on PC) it still causes fatigue and eventual pain. I would prefer the material cradle my wrist either by adding additional depth to the support material while softening it or again have a contoured shape.
Overall this works incredibly well and for somebody who put a ton of effort and money in the aesthetics of my PC master race set up with RGB everywhere and whatnot, having a simple and good looking wrist rest goes a long way to add to the look without detracting from any other element as those other "Gamer" wrist rests do. I love the thing and it is the best I have found however for my personal situation with wrist injuries I would love this company to produce one focused on fatigue prevention for those of folks with common PC use conditions.
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Reviewed in the United States on May 29, 2020
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