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Description
Calreticulin Recombinant Rabbit mAb (S-R353)Product Specification Host Rabbit Synonyms CRP55, Calregulin, Endoplasmic reticulum resident protein 60 (ERp60), HACBP, grp60, CALR, CRTC Location Cytoplasm, Secreted Accession P27797 Clone Number S R353 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC, ICFCM Reactivity Hu Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin
Product Specification
| Host | Rabbit |
| Synonyms | CRP55, Calregulin, Endoplasmic reticulum resident protein 60 (ERp60), HACBP, grp60, CALR, CRTC |
| Location | Cytoplasm, Secreted |
| Accession | P27797 |
| Clone Number | S-R353 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ICFCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | null |
| IHC-P | 1:2000 | null |
| ICC | 1:500 | null |
| ICFCM | 1:500 | null |
Background
Calreticulin is a multifunctional soluble protein that binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal (see inositol trisphosphate). Calreticulin is located in storage compartments associated with the endoplasmic reticulum and is considered an ER resident protein. Calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the Golgi apparatus.
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