SKU: 75291273678

Mouse IL-10 ELISA Kit(Plus)

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Description

Mouse IL-10 ELISA Kit(Plus)Product Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti mouse il 10 Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process; Il 10 Bound to solid phase

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse il-10  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process; Il-10  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and sample   The concentration of IL-10 was positively correlated. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-10 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 5.80 pg/ml

Detection Range: 31.3-2000pg/ml

Recovery Range: 86-108%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 10 (IL-10)It is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL-10 gene located on chromosome 1 and consists of five exons. IL-10 is mainly secreted by monocytes, while lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells, and partially activated T cells and B cells secrete less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - & kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.

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SKU: 75291273678

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Maria
Boise, US
★★★★★ 5
I recommend using this book. Read review.
Format: Hardcover
I recommend this book. Not only because it's a Dr. Sue's book, but because at the end of the school year. I ask my son's teacher to sign it. It's a sweet memory to have and it will be a sweet memory to look at once they graduate Elementary. It's a must. The price is great. Quality... Everything about it is great. Most of all.... Reading the their teacher's have to say about them. Is so sweet!
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Reviewed in the United States on May 23, 2026
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Mommy Sarah
Pawtucket, US
★★★★★ 5
Dr. Suess. Enough Said.
Format: Hardcover, Format: Hardcover
Welcome to the whimsical world of Dr. Seuss, where "Oh, the Places You'll Go!" isn't just a book—it's a roadmap to life's most thrilling escapades! If life were a rollercoaster, this book would be the front-row seat to the wildest ride of all. From the moment you crack open its vibrant pages, you're whisked away on an exhilarating journey filled with colorful landscapes, quirky characters, and nuggets of wisdom disguised in Seussian rhymes. It's a literary wonderland where imagination reigns supreme, and the words dance off the pages, pulling you into an adventure unlike any other. Dr. Seuss, the maestro of whimsy and wonder, takes you on a whirlwind exploration of life's ups and downs, encouraging you to embrace every twist, turn, and unexpected detour with unbridled enthusiasm. Whether you're just starting out on life's path or navigating its winding roads, this book serves as a beacon of hope, inspiration, and boundless possibilities. Now, let's talk about the humor woven into every verse—oh, it's a riot! Dr. Seuss's playful language and zany illustrations transform life's perplexities into delightful puzzles to solve. You'll find yourself chuckling at the absurdity of the situations and nodding knowingly at the profound truths hidden within the jest. And oh, the illustrations! They're a visual feast for the eyes, bursting with vibrant hues and fantastical landscapes that transport you to worlds beyond your wildest dreams. The whimsical characters and their expressions evoke emotions that range from sheer joy to contemplative reflection, leaving an indelible mark on your heart. But wait, there's more! This book isn't just for kids—it's a timeless gem that resonates with readers of all ages. Whether you're 8 or 80, the wisdom embedded in its verses speaks volumes about the human spirit's resilience, determination, and unwavering courage to conquer life's myriad challenges. In essence, "Oh, the Places You'll Go!" isn't merely a book; it's a celebration of life's rollercoaster, a symphony of laughter, and a profound reminder that the journey itself is the greatest adventure of all. So, buckle up, embrace the whimsy, and get ready to soar to unimaginable heights—you're in for an exhilarating ride! *Disclaimer: May induce spontaneous outbreaks of giggles and an insatiable thirst for adventure!*
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Reviewed in the United States on November 15, 2023
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Eve Allen
Boise, US
★★★★★ 5
Oh, the places you'll go! Book Review
Format: Hardcover
Perfect hardcover book for such an affordable price!
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Reviewed in the United States on May 25, 2026
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Destiny Evans
Phoenix, US
★★★★★ 5
Must have
Format: Hardcover
Great quality hard back book. I have purchased 4, one for each of my children and will eventually but 2 more. Highly recommend.
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Reviewed in the United States on May 18, 2026
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Donna K
Massapequa, US
★★★★★ 5
Makes a great graduation gift
Format: Hardcover
It's a classic! I bought it for my granddaughter's kindergarten graduation. I've asked her teacher to write a note in it.
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Reviewed in the United States on May 29, 2026

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