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Description
Mouse GGT ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 mU/mL). Then dilute to the following concentrations: 20 mU/mL, 10 mU/mL, 5 mU/mL, 2.5 mU/mL, 1.25 mU/mL, 0.625 mU/mL, 0.3125 mU/mL, 0.000 mU/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 20mU/mL standard working solution into the first EP tube and mix thoroughly to make a 10mU/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a gamma-glutamyl transpeptidase (GGT) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of gamma-glutamyl transpeptidase (GGT) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse gamma glutamyl transpeptidase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Gamma-glutamyltransferase 1 (GGT1), also known as CD224 (cluster of differentiation 224), catalyzes the transfer of a glutamine group to various amino acid and dipeptide acceptors. This isomerase consists of a heavy chain and a light chain, which are derived from a precursor protein and are present in tissues involved in absorptive and secretory processes. This enzyme is a member of the gamma-glutamyltransferase protein family, many members of which have not been fully characterized. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 mU/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.7 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 1
Sunveza MacBook Pro Charger: A Game-Changer on a Budget!
Man, where was this Sunveza charger when I first got my MacBook Pro? I'm the type who'd forget my head if it wasn't attached, and yup, I did it – forgot my charger on my first day bringing the new MacBook to the office. Enter Sunveza with its high-efficiency and surprisingly affordable solution.
First off, this thing charges FAST. Talking about going 0 to 80% in what feels like a blink. And the compatibility? Spot on. Whether you're rocking a MacBook Pro, Air, or even an iPad, this charger's got you. Plus, the multiple security features? A major bonus. Peace of mind knowing my gadgets are safe from overcharging or short-circuits.
Now, if we're comparing aesthetics, yeah, it doesn't have that Apple "heft." But honestly, does that matter? It's like comparing a sports car by the weight of its keys. What matters is performance, and Sunveza delivers.
I stumbled upon this gem after a less-than-stellar experience with a pricey off-brand from Target. Sunveza's charger is just the right size, doesn't sag from the outlet, and feels just right. The only tiny hiccup? The cable can be a smidge finicky sometimes, needing a gentle push to snugly fit. But considering the overall value and the fact that I could always grab a new cable if needed, it's a minor gripe.
In short, if you're in the market for a MacBook charger that won't break the bank and performs like a champ, Sunveza's got your back. Highly recommend! 🌟🌟🌟🌟🌟
EDIT: Wow okay... so things were working great for several months but then I started having issues with this charger and it would intermittently start and stop charging. The charger block itself would also get painfully hot to the touch. I had been using this product for less than a year when I started having these issues. I tested this out with other devices and outlets and witnessed the same behavior. I also took my MacBook to the Apple Store and they informed me that my battery was damaged, likely because of this charger. In light of this, I can no longer recommend the Sunveza charger.
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Reviewed in the United States on August 26, 2023
★★★★★ 5
Supercharge Your Devices in Record Time
I recently got my hands on this speedy 118W USB C charger, and boy, has it been a lifesaver for my tech-filled lifestyle! From juggling work on my MacBook Pro to catching up on my favorite shows on my tablet, having a charger that keeps up with my demanding schedule is such a relief. This charger isn't just fast; it's like a pit crew for your devices, getting them back to full power in no time.
What truly stands out is the efficiency and versatility of this charger. I was amazed at how quickly it charged my MacBook Pro—fully juiced up in just over an hour! Plus, the fact that it can charge my phone to 80% in just 20 minutes is a real time-saver. No more waiting around tethered to an outlet. The wide compatibility means I can use it with all my USB-C devices, from my laptop to my tablet and even my friend's Samsung Galaxy. It automatically adjusts the power output, which means I don't have to worry about frying my devices. How cool is that?
The peace of mind this charger provides is another big plus. With built-in features like over-current and temperature protection, I know my gadgets are safe while charging. It's clear that a lot of thought went into the design, using high-quality materials and smart technology to ensure a long service life. Plus, the included charge cable is a nice touch—no need to hunt for a compatible one!
In short, if you're on the lookout for a reliable and efficient charger that covers all your devices, look no further. This charger has made my tech life so much easier and worry-free. And if you ever have any questions, their customer service team is just an email away, ready to help. It's a solid investment in keeping all your gadgets powered up and ready to go.
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Reviewed in the United States on June 30, 2025
★★★★★ 4
Adapter doesn’t get super hot
I have a 2016 Mac Book Pro that I was convinced was broken, it just needed a new charger (lol) this works great for my laptop, phone, and iPad. Perfect length and the brick doesn’t get super hot when I use it.
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Reviewed in the United States on May 8, 2026
★★★★★ 5
Useful
It looks a bit thinner than the original Apple one, but it performs just as well in use. It charges very quickly, and the cable is also very sturdy.
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Reviewed in the United States on May 21, 2026
★★★★★ 5
LOVE THEM🩷
Love these charges so much and have been using them for years! However, each charger only lasts about 6 months. I think they are worth it though and very good. It comes with two so you only need to buy a new pack once a year. Most chargers nowadays don’t last anyway. These charge fast and are very long. Also love the color options, I have mine in pink🩷
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Reviewed in the United States on April 15, 2026