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Description
Endo S2Product Specification Species Streptococcus pyogenes Synonyms Endo beta N acetylglucosaminidase EndoS2Endoglycosidase S2EndoS2 Expression System E. coli Molecular Weight 93 kDa (Reducing) Purity 95% by SDS PAGE Conjugation Unconjugated Tag His Tag Physical Appearance Liquid Storage Buffer 20 mM Tris HCl50 mM NaClpH 7. 5 @ 25C Stability & Storage Store at 25 ~ 15 for 2 years Reference [1] Sjgren Jonathan, et al. "EndoS and EndoS2 hydrolyze Fc glycans
Product Specification
| Species | Streptococcus pyogenes |
| Synonyms | Endo-beta-N-acetylglucosaminidase EndoS2、Endoglycosidase S2、EndoS2 |
| Expression System | E.coli |
| Molecular Weight | 93 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris-HCl、50 mM NaCl、pH 7.5 @ 25°C |
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference | [1] Sjögren Jonathan, et al. "EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans." Glycobiology 10:1053-1063. |
Background
Endo-S2 is an endo-n-acetylglucosaminase derived from streptococcus pyogenes that excises the glycosidic bond between the two innermost n-acetylglucosamines of the glycoprotein n-linking sugar chain. It is effective for high mannose type, hybrid type and double antenna complex type. Can be made for all human IgG, as well as mice, rats, monkey, goat, sheep, cattle and horse IgG antibody glycosyl.
Components
Storage Solution : 200U/ul Endo S2 、20 mM Tris-HCl, 50 mM NaCl. (pH 7.5 @ 25°C) 10*Reaction Buffer： 500 mM sodium acetate (pH 6.0 @ 25°C)
Protocol
1. Combine 100 µg of native IgG, 1 µl of 10*Reaction Buffer and H20 (if necessary) to make a 10 µl total reaction volume.
2. Add 1 µl EndoS2.
3. Incubate reaction at 37°C for 1 hour
Guidelines
Please avoid repeated freeze-thaw cycles.
Unit Definition
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