Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 8 - Aug 13
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human XBP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody for X-Box Binding Protein 1 (XBP1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of X-Box Binding Protein 1 (XBP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human for X-Box Binding Protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | X-box binding protein 1, also known as XBP1 or TREB5, is a protein encoded by the XBP1 gene. The XBP1 gene is located on chromosome 22, while a closely related pseudogene has been identified and mapped to chromosome 5. This protein is a transcription factor that regulates the expression of genes important for the normal functioning of the immune system and cellular stress responses. It is a bZIP domain-containing transcription factor. It was first identified by its ability to bind to Xbox, a conserved transcriptional element in the human leukocyte antigen (HLA) DRα promoter. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.4 ★★★★★
Based on 26 reviews
Sort
Product Reviews
★★★★★ 5
A Must-Have for any Final Fantasy Fan
Format: Hardcover
GeminiGeek's Honest Review!
If I had to review this Ultimania Volume 1 with a single word, I'd simply say: "FANTASTIC!". This first volume of the Final Fantasy Ultimania books covers the first six Final Fantasy games, and has an enormous amount of details and content. Each game has it own individual section, complete with images of each character's classic sprites, and even their official Yoshitaka Amano art work as well. Aside from being visually appealing, the book is overflowing with unique story details, character bios, and even an encyclopedia of items and monsters! This volume of the Ultimania series is thick, it is huge, but not to the point of being a pain to carry around or hold. If you enjoy any of the six Final Fantasy games that this volume covers, you'll find something to love in this book, there is just so much information and artwork contained within this tome.
This is a must-have for anyone who enjoys the Final Fantasy series, I can't recommend it enough. As a long-time Final Fantasy fan myself, I love this book, and I can't wait for Volume 2 to come out this fall.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 26, 2018
★★★★★ 5
This is the book I've been waiting for!!!
Format: Hardcover
This book has a ton of great information for fans of the first 6 Final Fantasy games. It includes artwork, sketches, maps, computer renderings, and more! Check out the video flip through of the book that I added. If you are a fan, you won't be disappointed!
Note: many characters, monsters, and locations are shown with Japanese language (Katakana or Hiragana most often - sometimes Kanji) with a Romaji translation (such as Kokaturisu for Cockatrice) and a final English translation. Note that much of this book is based off multiple versions of the games - often the PSP / PS1 versions for 1, 2, 4, 5 and DS for 3. Just a note because translations are different for each version. Also some of the sprites shown in character models are from later releases, not original. But it is certainly forgivable since they often show the sprites for multiple versions.
I am just pawing through it non-stop. I feel like a kid when I got the first Final Fantasy and literally wore out the map/monster chart that was included!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 24, 2018
★★★★★ 5
Great gift for Final Fantasy Enthusiasts
Format: Hardcover
My brother in law has played many of the original Final Fantasy games and has always enjoyed the art style. My husband purchased this book for him as a birthday gift and my brother in law has thoroughly enjoyed flipping through this book. The hardback cover is very sturdy and the entire book is beautiful with interesting character facts throughout.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 25, 2024
★★★★★ 5
Even better than expected
Format: Hardcover
This is a really good quality book both in form and content. Its pretty big both in size and thickness, its actually taller than i thought and is very well made with beautiful print. If you are a fan of Final Fantasy or a fan of videogame artwork its a great item to add to your collection. I'd love to get the other two volumes as well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 22, 2024
★★★★★ 5
An interesting read for fans of the classic series.
Format: Hardcover
The Ultimania is a nice collection of background info and original artwork for the series, and this book details the first six entries that appeared on Nintendo systems between 1987 and 1994. For fans of the 8-bit era, you may be a bit disappointed to find their sections a bit light on content, but they do feature gorgeous artwork from Yoshitaka Amano, Akihiko Yoshida, and other artists who worked on the remakes for the Wonderswan and PSP versions. There is also some neat background info like notes and outlines from the titles early development.
The real meat of the book comes from the 16-bit era entries which detail some interesting info not usually seen, such as artwork of the characters by the staff, dungeon design details, and a few minor cut content. If you're a serious fan of the franchise and can't read Japanese making the 20th and 25th anniversary Japanese Ultimania books nothing more than an art book for you, then this is a great buy for super fans.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 12, 2019
recommand products
Eibach ERS 7.00 inch L x 2.50 inch dia x 400 lbs Coil Over Spring - 0700.250.0400
85.00
PosiQuiet 99-04 Honda Odyssey / 01-02 Acura MDX Extended Wear Front Brake Pads - 106.07930
70.30
Clutch Masters 07-10 Nissan Altima/Sentra SE-R FX350 Sprung Fiber Tough Clutch Kit - 06074-HDFF-RH
318.75
Cusco GVB Impreza 5 Passenger 6P DASH AROUND Roll Cage D1 Chro-moly YELLOW (S/O / No Cancel) - 195 261 B
652.50
Enkei PF01 17x7.5 5x114.3 45mm offset 75mm Bore Dia Silver Wheel - 460-775-6545SP
142.65