Pay in installments of $135.38 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 13 - Aug 18
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human Ovarian Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
||||||||||||||||
| Description | Composition:
|
||||||||||||||||
| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 5 reviews
Sort
Product Reviews
★★★★★ 3
Carefully measure your size
Size: Large, Color: Ocean Blue
So difficult to get over my shoulders I guess it's a little small for me. Comfortable but I don't want to take it off or put it on
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 1, 2025
★★★★★ 1
Larger than life
Size: X-Large, Color: Ocean Blue
The fit is large! So go down a size. It’s soft and seems like good quality, but absolutely huge
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 16, 2025
★★★★★ 5
Very good quality sports bra.
Size: Large, Color: Navy
Very good sports bra. It is high quality and made well. It is a full coverage bra which gives it good support but also makes it a little hot especially in warmer temperatures but at the same time it is very nice for cooler temps.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 20, 2026
★★★★★ 4
Supportive and Comfortable but Size Down!
Size: X-Large, Color: Navy
Very supportive for the big chested girlies! Material is comfortable and sweat wicking. I’d recommend sizing down for a snug fit.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 25, 2026
★★★★★ 5
Nice fabric, comfortable, easy maintenance
Special Size: 27.5" Full Length, Size: Large, Color: Taupe, Special Size: 27.5" Full Length, Size: Large, Color: Taupe
I ordered a medium and they’re actually a size large. And they are just too large. I should’ve gotten the small, as recommended I thought I was ordering medium and I think I would’ve been OK with a medium, but where I usually wear a small and they sent me a large. It was just ridiculously large. They are lightweight and they are warm. I’m a senior and I’m thin so I get cold very easily especially in early morning and evening, so I like to wear them in the morning for my walks/yoga , they also work nicely under a longish skirt so it’s not so sheer… will definitely get more but will go with the small size. Just for reference, I’m 5 foot seven I usually wear a small or a size 5/6 usa size so these were too large, particularly in the waist just overall they were too large, but I think even if I got a small size they’re still gonna be kind of large in the stomach. I have an hourglass shape just for reference, but they are very comfortable, and I love the fabric. I am keeping them because I like the fabric and I really need them. I just will not wear them out of the house right now because they’re not fitting properly but next go round we’ll get the right size. Price was right they were having a sale so it was a bit of an impulse buy. But hello it was only 10 bucks! so that’s why it’s not worth sending it back for me. Overall, very nice quality and would recommend. Cats like the fabric too! 😸
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
recommand products
Women's Cologne Carnival Print Casual Waffle Long Sleeve T-Shirt
27.99
Men's Retro Business Style Casual Work Trousers
39.99
XClutch 67-69 Chevrolet Camaro RS 5.3L Stage 1 Sprung Organic Clutch Kit (XKBU28001-1A)
171.54
Women's Walk For Peace Monks With Aloka Dog Printed Sweatshirt
34.99
VIS Racing Carbon Fiber Hood VS 2 Style for Honda Civic Type R 2016-2020(16HDCVCTYRVS2-010C)
700.50