Human IL-22 High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-22 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-22 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-22 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-22 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.67pg/ml

Detection Range: 6.25-400 pg/ml

Recovery Range: 92-117%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 22 (IL-22) is an IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26)As a member of cytokines, it is an effective regulator of cellular inflammatory response. It uses il-10r2 together with other members of this family, IL-10, IL-26, il-28a/b, and IL-29, in cell signaling. IL-22 is produced by activated DCs and T cells, which initiate innate immune responses against pathogenic bacteria, especially epithelial cells derived from the respiratory tract and gut. The plasma of psoriasis patients showed strongly elevated IL-22 levels, which correlated with the severity of the disease. In T cell-mediated hepatitis induced by concanavalin A (Con A), IL-22 can play a protective role as a survival factor of hepatocytes. With ifn-γ In contrast to IL-17, there is a large amount of IL-22 in the blood of patients with Crohn's disease