Human IgA ELISA Kit

Testing Principle：
This kit uses double antibody sandwich ELISA.Specific anti-human IgA capture antibody is prepackaged on a high affinity conjugate plate.  Standard substance, test sample and detection antibody labeled with horseradish peroxidase were added into the HRP-plate well. After incubation, IgA in the sample was combined with solid phase antibody and detection antibody.  After washing, color substrate TMB is added to protect the color from light.  The depth of the color reaction is proportional to the concentration of IgA in the sample.  A termination solution was added to terminate the reaction and the absorbance was measured at 450 nm (correction wavelength 570-630 nm).  

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：pre-coated 96-well plate,Standards,HRP-labeled IgA test antibody, test buffer, TMB color substrate, washing solution, stop solution, sealing plate membrane and instruction manual  

Sensitivity：0.35 ng/mL

Detection range：3.13-200 ng/mL

Recovery rate：90-108%

Storage：2-8℃

The standard curve：

Background：

Immunoglobulin A(IgA) is an antibody that plays an important role in mucosal immunity. Its heavy chain is the alpha subtype. There are two subclasses of IgA, IgA1 and IgA2, which exist as secretory IgA(sIgA) dimer. In mucosal secretions, SIgA is the most important immunoglobulin, which is less present in blood. The secretory component of SIgA protects immunoglobulin from proteolytic enzyme degradation, so SIgA can exist in harsh gastrointestinal environment and inhibit microbial reproduction in body secretions. SIgA also inhibits the inflammatory effects of other immunoglobulins. The activation and regulation of complement system by IgA is weak.