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Description
NA/LE Syrian Hamster anti-mouse CD28 mAbProduct Specification Host Syrian Hamster Antigen CD28 Immunogen C57BL 6 mouse T cell lymphoma EL 4 cells Clone Number 37. 51 Antibody Type Syrian hamster mAb Isotype Syrian Hamster IgG2 Application in vitro T cell stimulation activation, in vivo CD28 blockade Reactivity Ms Purification Protein G Concentration Lot specific* (generally 4 to 11 mg ml)* Molecular Weight 150 kDa Purity >95%(Determined by SDS PAGE) Endotoxin <2EU mg (<0. 002EU g), 0. 2 M
Product Specification
| Host | Syrian Hamster |
| Antigen | CD28 |
| Immunogen | C57BL/6 mouse T cell lymphoma EL-4 cells |
| Clone Number | 37.51 |
| Antibody Type | Syrian hamster mAb |
| Isotype | Syrian Hamster IgG2 |
| Application | in vitro T cell stimulation/activation, in vivo CD28 blockade |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | Lot specific* (generally 4 to 11 mg/ml)* |
| Molecular Weight | 150 kDa |
| Purity | >95%(Determined by SDS-PAGE) |
| Endotoxin | <2EU/mg (<0.002EU/μg), 0.2 μM filtered Determined by LAL gel clotting assay |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH6.0 containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Background
This antibody recognizes the same epitope as clone 37.51.
This monoclonal antibody reacts with mouse CD28, a 45 kDa costimulatory receptor and a member of the lg superfamily, CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 augments lL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. This antibody has been shown to stimulate the proliferation and cytokine production by activated T and NK cells and provide a costimulatory signal for CTL induction.
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