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Description
Mouse hs-CRP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against hypersensitive C-reactive protein (hs-CRP). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the level of hypersensitive C-reactive protein (hs-CRP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse hypersensitive C Reactive Protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hypersensitive C-reactive protein (hs-CRP) is a type of C-reactive protein in plasma, also known as high-sensitivity C-reactive protein. Its clinical guidance primarily focuses on cardiovascular disease and kidney transplantation. hs-CRP, a nonspecific marker of the acute phase of systemic inflammatory response produced by the liver, is one of the strongest predictors of cardiovascular event risk. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
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Product Reviews
★★★★★ 5
Excellent Price & Function!
Size: No Size, Style: 4V Screwdriver_Twist 2.0 2pc Set
An excellent power screwdriver for assembly work where high torque is not needed. I haven't stripped any screw threads yet. The battery lockout feature is nice especially if it's bumping around in a tool box. It feels good and the switch works good too. The battery indicator LEDs work good too. The adjustable angle on the end works good too. Watching it turn through the clear windows is a neat function too. It works better than the Ryobi screwdriver it replaced. The torque is good without having too much torque for lighter work. It looks nice and feels of really good quality. The battery life appears to be good as well. I would buy it again when needed. Excellent price!
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Reviewed in the United States on November 24, 2025
★★★★★ 5
Great tool
Size: No Size, Style: 4V Screwdriver_Twist 2.0 2pc Set
This thing is exactly what I wanted, now everybody saying that it lacks power or anything else, doesn’t understand Tools in my opinion that’s what an impact drivers for. This thing isn’t meant to lag screws into a piece of wood. But this thing is perfect for anything you would just need a normal screwdriver for like installing door handles or outlets or light fixtures or furniture, etc. I find it has plenty of torque, and it could fit into most areas with the swivel design. And feels like a good tool doesn’t feel cheap. And unlike the other ones that have this weird twist motion to activate it, this one has a Forward and reverse button which I prefer.
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Power your way on next job. Just buy it!
Size: No Size, Style: 4V Screwdriver_Twist 2.0 2pc Set
I have every tool known to man. I have tools to fix other tools. Most of them are rubber coated for safety. But I didn’t have a powered screwdriver. Not sure why. This thing is great, I had a project where I had to take apart a Kirby vacuum and this really sped the process, looking forward to using it on 1 million other projects, versus my impact driver, which I used on most everything. Torque seems great, even loosens tight screws, and I like the 45° bend to hold the screwdriver. Seems to hold a charge very well, and it feels lightweight in hand. Not sure why I didn’t buy one 20 years ago. Good value, as I bought it on sale.
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Reviewed in the United States on March 18, 2026
★★★★★ 4
Perfect for small home projects
Size: No Size, Style: 4V Screwdriver_Twist 2.0 2pc Set
Good for anyone who is looking to have a small bit and doesn't really use a manual screwdriver to match or use a heavy duty drill. Good for this. Getting small tasks then but it can also screw into wood. I did test it and it's pretty well working not as fast as your regular drill can. The power is pretty high but no it does lose percentage over time. The grip of it's nice and comfortable and easy to use. Seems like it has a magnetic head to help keep your screws together is about the size of a hand but is arguably still bigger? Haven't even lost any pieces to it. Pretty high durability and good for its size
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Reviewed in the United States on September 30, 2025
★★★★★ 5
Heavy duty tool for light work! GREAT VALUE IMHO
Size: No Size, Style: 4V Screwdriver_Twist 2.0 2pc Set, Size: No Size, Style: 4V Screwdriver_Twist 2.0 2pc Set
Great tool to add to the box. I had this in my cart as I was looking for something small that was electric and had a flexible head, mostly for auto (being able to get all but the torque in tight spaces) and also at work where I’m constantly using a driver and wanted to save my wrists some work. There are others out there, but most are Amazon only brands, which come from China and are hard to get good customer service if there’s an issue. You buy something and have an issue a year later and find that the company either doesn’t exist or has changed their name. It’s a tactic. I buy USA made when it’s available, and buy American brands like Skil and Craftsman, etc. as you can trust they’ll make things right if needed. Anyway, I love this thing. I use it multiple times a day, but it’s about 6 bolts in, work for a few hours, 6 bolts out, then back in, and so on. So I’m not running it non stop. It’s a $20-30 tool, so I don’t expect it to last with some crazy heavy use on a job site. I don’t use the bit it came with. I use this exclusively with socket driver bits. Mostly a 1/2” tool get the light work done, then pull out the torque wrench and finish it. I saw this on sale in town for the same price, so I bought one for my 17yo son who is always wrenching on stuff. Great tool to have for getting the lighter thousand turns out of the way. It would probably last forever if used around the house and charged by the book. I’ve only recharged it once and at the rate I use it one charge lasted a couple weeks. It is built very well, and has heavy-duty rubberized grip around with the body of a thicker, power tool type plastic. 100% recommended to anyone and everyone. This is the type of thing any man that actually works and fixes things around the house and garage/shop, young or old, would appreciate.
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Reviewed in the United States on April 17, 2026