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Description
MTT Cell Proliferation and Cytotoxicity Assay KitProduct Specification Usage 1. Place the cell plate in the sterile operating table. 2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg ml MTT solution. It can be used after preparation, or stored directly at 20 in the dark from light, or stored at 20 in the dark from light after appropriately packaging as needed.] 3. Put the cell plate back into
Product Specification
| Usage | 1. Place the cell plate in the sterile operating table. 2. Add 10% volume of MTT solvent to each well of the cell plate. [Preparation of MTT solution: Dissolve 25mg of MTT with 5ml of MTT solvent to prepare a 5mg/ml MTT solution. It can be used after preparation, or stored directly at-20 ℃ in the dark from light, or stored at-20 ℃ in the dark from light after appropriately packaging as needed.] 3. Put the cell plate back into the incubator and continue to incubate for 3-4 hours. When doing comparative experiments, the incubation time should be consistent. 4. After incubation, take out the cell plate from the incubator and dissolve it according to the following method to produce MTT formazan crystals. (1) If it is an adherent cell, discard the culture medium. Add the same amount of Formazan Solvent as the initial volume. The volume of dissolved fluid may vary per well, but the total volume must be consistent for comparison. (2) If the removal of non-adherent cells or culture medium will cause the loss of MTT formazan, add the same amount of lysate as the initial volume directly to the culture medium. (3) Readings must be completed within 1 hour after addition of Formazan Solvent. 5. Gentle shaking in a shaker can enhance dissolution. In some cases, particularly when the cell density is high, repeated pipetting is required to completely dissolve the MTT formazan crystals. 6. Spectrophotometer to measure OD570 and reference wavelength OD690. 7. The 96-well plate can be detected on a microplate reader equipped with a suitable filter. 8. Other types of multi-well plates need to be spectrophotometrically detected with a cuvette or plate reader of appropriate size. |
| Beilstein | 0 |
| Synonym | MTT Cell Proliferation and Cytotoxicity Assay Kit,MTT kit |
| Description |
The MTT assay, also known as MTT colorimetry, is a method to detect cell survival and growth. The detection principle is that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline Formazan and deposit it in cells, while dead cells do not have this function. Within a certain range of cell numbers, the amount of MTT crystals formed is directly proportional to the number of cells. The greater the absorbance, the stronger the cell activity, indicating that the drug toxicity is less. This method has been widely used in the activity detection of some bioactive factors, large-scale anti-tumor drug screening, cytotoxicity test and tumor radiosensitivity determination. It is characterized by high sensitivity and economy. Components: MTT (25mg), MTT solvent (5ml), Formazan solution (55ml)
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| PubChem CID | 0 |
| General Notes | 1. Since a 96-well plate is used for detection, if the cell culture time is long, we must pay attention to the problem of evaporation. On the one hand, since the circle around the 96-well plate is the easiest to evaporate, you can discard the circle around the plate and add PBS, water or culture medium instead; On the other hand, a 96-well plate can be placed close to a water source in the incubator to alleviate evaporation. 2. If the test compound used can react with MTT, centrifuge first and then discard the culture medium. Carefully flush it with PBS for 2 to 3 times, and then add the culture medium containing MTT. 3. The production of Formazan is not only proportional to the number of living cells, but also affected by the action time. Therefore, when there are many samples, the measurement results may change with time. 4. This product contains carcinogens, avoid contact with skin and eyes, please operate carefully. For your safety and health, please operate in a lab coat and disposable gloves. 5. This product is only used for scientific research and cannot be used clinically or diagnostically. |
| Storage Temp. | Stored at 2 ~ 8 ℃, protected from light, valid for 12 months. |
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4.5 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 4
Long lasting
Color: Dogwood & Calming, Size: Medium
My dogs love these bones.
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Reviewed in the United States on March 25, 2026
★★★★★ 5
Puppy loves these sticks!
Color: Dogwood & Calming, Size: Medium
Dogs love these sticks! Must smell and taste like real wood because it keeps my puppy from bringing real sticks in the house from outside. She is a heavy chewer and these sticks keep her occupied. We are on our 2nd set.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Great value for the price, highly recommend
Color: Dogwood & Calming, Size: Medium
Our dog loves these and chews them down to nothing. They are a great value for the money and the use your dog gets out of them. The size is just right for our doberdor.
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Reviewed in the United States on May 20, 2026
★★★★★ 3
Our dog loves it but it wasn’t durable enough to last
Color: Dogwood Jack, Size: Large, Color: Dogwood Jack, Size: Large
Our lab took to it as soon as we gave it to her. Perfect size for our 80 lb yellow lab.
However, now that it’s in about 10 days, she’s able to break off chunks from this bone that are dime size or larger, and we have to throw it away
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Reviewed in the United States on May 1, 2026
★★★★★ 5
No problems after a year and a half
Color: Dogwood & Calming, Size: Medium
Have been regularly buying these for my corgi for about a year and a half. The 2 pack lasts about a month or 2 each time. Pieces break off in small enough pieces that they safely pass through his digestive system. He gnaws each one down to about 1/4 the original size before we take it away and give him a new one. He continues to have regular bowel movements and has a healthy appetite so I can’t imagine any problems arising after so long.
I have tried other alternatives and every other similar chew breaks off in larger pieces and didn’t feel comfortable letting him chew/eat them. I noticed there are a few 1 star reviews saying their dogs got sick off these but after a year and a half I have had 0 issues with this product. I guess it depends on the dog and how big of pieces they are swallowing. My dog ingests pieces about the size of a grain of rice so just pay attention and you should be fine.
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Reviewed in the United States on February 18, 2026