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Description
Human HPEPT1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with peptide transporter 1 (HPEPT1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of peptide transporter 1 (HPEPT1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Peptide transporter 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Hydrogen ion/peptide transporter 1 (HPEPT1), also known as solute carrier family 15 member 1 or peptide transporter 1 (PEPT1), is a protein encoded by the SLC15A1 gene. It is a solute carrier for oligopeptides. It functions in a proton-dependent manner in renal tubular oligopeptide reabsorption and in the intestine, thus acting as a cotransporter. It is localized to the brush-border membrane of the intestinal epithelium and mediates the uptake of di- and tripeptides from the lumen into enterocytes. This protein plays an important role in the uptake and digestion of dietary protein. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
IBS C must have!
I have been suffering with OBS C for a very long time now and my doctor through Amazon Care (if you haven't downloaded that app yet, do it now) recommend that I take magnesium and the Align probiotic with breakfast and Metamucil in the evening before bed. I am finally feeling better and have not had any issues using the restroom! I recommend for sure. The taste is meh but I would rather down something that doesn't taste great as long as I feel better anyway!
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Reviewed in the United States on June 2, 2022
★★★★★ 5
Great for my tidy mind. Now the cabinet is organized and clean. Space saving too
Size: Left Facing, Size: Left Facing
Easy install. Glides nicely holds the jars without much movement. The labels are wonderful. I am super pleased. We bought 4 left facing for our cabinet. Not done replacing all the jars and such. But you can see a good idea how it will be. The fit out needs perfectly and looks great. I really like how neat and good visibility too. They seem sturdy with all the weight of the filled bottles also.
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Reviewed in the United States on March 1, 2026
★★★★★ 5
Great space saver, keeps everything organized.
Size: Left Facing, Size: Left Facing
I am soooo happy with this purchase! I’ve been researching lots of spice racks to see which ones would best suit my needs. This definitely fits the bill.
This product was perfect. It includes everything you need to organize your spices: the rack, screws, stickers, bottles, and a white gel pen to write additional spice names. The rack is heavy duty and sturdy. Once anchored to your cabinet, there’s no way it’s falling out.
The bottles are a good size and you can transfer standard store bought spices directly into the bottles. An additional asset is that the bottles come with a shaker spout that has two options for use. It can easily snap off and on when refilling.
The stickers are well thought out. They’re good quality labels and can easily be repositioned. I accidentally had an edge stick to the middle of a bottle and was scared that it wouldn’t come off. I was happy that it lifted up cleanly and didn’t wrinkle the sticker.
I didn’t have a need to write a lot of new names, except for two so far. Using the pen included, I wrote labels for Dashi and Seasoning-Arrachera. I tried to match the font of the labels and I think I did a pretty good job. You wouldn’t notice it was hand written unless you really stared at it. The white pen is great and so easy to write with. I didn’t have to go over any letters. One pen stroke was enough.
It also came with labels that you can place on the side of the rack for easy identification. I haven’t put them on mine yet because I still need to buy two more racks to store all my spices. Then I can get them all organized the way I want.
This is such a space saver. One of my absolute favorite purchases!
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Reviewed in the United States on March 24, 2025
★★★★★ 5
Absolutely worth the money!
Size: Left Facing, Size: Left Facing
I can't say enough about these spice racks! I have kept my spices in a corner cabinet on lazy Susans for the last 25 years and that's worked great, but it did not have much curb appeal and some spices were in the middle of the caddies so I would have to pull the front ones off to get to the middle ones. Corner cabinets are tricky and can be such a waste of space, but I made it work. But now it doesn't just work, it sings! My husband bought me 4 units and they fit perfectly in the space. Whoever designed these racks thought through EVERYTHING. The labels are well organized with plenty of obscure herbs and spices as well as all the common ones, and some blank stickers for the products they don't have labels for. There is a list of all the spices and which page you will find the label. There are also small corresponding labels to put on the ends so you know which rack has your spice (I haven't put those on yet because I want to make sure I have everything where I want it first). Since I have 4 sets of labels, I used some for labeling the baskets where I have the extra spices for refills. The racks are sturdy and easy to install. These would also work well in cabinets that are not corners - you would pull them straight out and it would make it easy to access all of your spices. If I could give this 10 stars, I would.
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Reviewed in the United States on December 28, 2025
★★★★★ 5
Fabulous!
Size: Left Facing, Size: Left Facing
I wish I had remembered to take a picture before I emptied my spice cabinet. Actually, on second thought, it would be embarrassing to share. I have two of these sturdy slide-out spice racks and could use another one. The racks were not hard to install, slide smoothly, look great, and my spices are in order! I was able to fit two of them on one side of my cabinet. I love the jars and labels. I use some spices much more than others so I put the matching labels on the large containers of these spices and keep them on the top shelf. I would definitely buy these again!
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Reviewed in the United States on February 15, 2026
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