SKU: 9818138884

Human TNF-α High Sensitivity ELISA Kit

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Description

Human TNF-α High Sensitivity ELISA KitProduct Specification Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte derived; TNFA; TNF A; TNFalpha; TNF alpha; TNF alphacachectin; TNFATNF, macrophage derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor alpha Description Testing Principle This

Product Specification

Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Description

Testing Principle:
This kit uses double antibody sandwich ELISA.Specific anti-human TNF-α Capture Antibody is precoated on a high affinity conjugate plate.  Standard substance, sample to be tested and biotin labeled detection antibody were added successively into the plate well of the enzyme label. After fully shaking and mixing, the samples were placed at room temperature for a 2-hour incubation process. The TNF-α in the samples was bound to the solid phase antibody and detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of color reaction was positively correlated with the concentration of TNF-α in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  


Detection type:Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity:100 μl

Kit composition:pre-coated 96-well plate,Standards,Standard, TNF-α Detector Antibody, Standard Diluent, Detector Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substrate, Washing Solution, Termination Solution, SA-HRP, Sealing Plate Mask and Manual  

Sensitivity:0.16 pg/mL

Detection range:1.56-100 pg/mL

Recovery rate:100-108%

Storage:2-8℃

The standard curve

 

Background:

Tumor necrosis factor α(TNF-α), also known as cachexin and TNFSF1A, is an adipokine that is involved in systemic inflammation and is one of the cytokines that stimulate acute phase responses. It is produced mainly by activated macrophages, but can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It has a variety of regulatory functions in the immune response, but also can be used as a potential pyrogen. In response to stimuli (infectious agents or tissue damage), TNF-α circulates throughout the body, activates neutrophils, alters the properties of vascular endothelial cells, regulates metabolic activity in other tissues, and exhibits tumor-killing activity by inducing local coagulation. TNF-α plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information indicates that TNF-α is also involved in the pathogenesis of AIDS. The determination of TNF-α is also useful for transplantation studies.  

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SKU: 9818138884

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Anahi
Alexandria, US
★★★★★ 5
BUY THIS!
Color: Black
Got this as a gift for my stepdad- he loves it!
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Reviewed in the United States on February 1, 2026
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RK
Charlottesville, US
★★★★★ 3
Not the quality I expected
Color: Brown
Good but not the quality I expected
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Reviewed in the United States on December 27, 2025
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B.Nicole
Port Orchard, US
★★★★★ 5
Perfect Drawer Organizers—Love the Assorted Sizes
Color: Clear
I really love these drawer organizers. The quality is great, and the assorted sizes are exactly why I decided to purchase them. I used a few inside my vanity to help keep everything more organized, and they work perfectly for separating smaller items without everything getting cluttered together. I like that there are multiple sizes included because it makes it easy to customize the layout for different drawers and products. One feature I really appreciate is the non-slip bottom. They stay in place well and don’t slide around every time I open and close my drawers, which makes a huge difference. The clear plastic design also makes it easy to quickly see where everything is without digging through the drawer making a mess. They feel sturdy and durable, not flimsy like some other organizers I’ve tried. I also like that they can be stacked when not in use, which helps save space. Best for: vanities, makeup organization, bathroom drawers, office supplies, and general home organization Overall, these are practical, well made, and really help make drawers look cleaner and more organized. Definitely happy with my purchase.
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Reviewed in the United States on May 11, 2026
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John Book
Dallas, US
★★★★★ 5
Clear, Stable, and Surprisingly Better Than IKEA
Color: Clear
I didn’t expect to be this impressed by drawer organizers, but the Vtopmart 25 PCS Clear Plastic Drawer Organizers really delivered. The non-slip silicone pads actually work — once placed, the trays don’t slide around at all, even when opening and closing drawers frequently. That alone makes a huge difference in daily use. What I love most is how much space they save while keeping everything clearly visible. The clear plastic makes it easy to find what I need at a glance, and the variety of sizes lets me customize each drawer instead of forcing everything into awkward layouts. All the organizers have a solid, usable depth, so items don’t tip over or feel cramped. Cleaning is effortless — a quick wipe and they look new again. Compared to IKEA organizers I’ve used before, these feel more practical, easier to maintain, and honestly more flexible. Add in the great price for a 25-piece set, and this feels like one of those simple purchases that quietly makes everyday life more organized and less frustrating.
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Reviewed in the United States on February 23, 2026
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Reviews by Christopher
Bozeman, US
★★★★★ 5
Keeps things organized
Color: Clear
These are worth buying. They can store so many things. We are using it for our vanities, and they work amazingly. They are super sturdy, and the clear plastic gives them a clean look. These are very helpful to have.
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Reviewed in the United States on April 5, 2026

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