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Description
MCM3 Recombinant Rabbit mAb (SDT-048-10)Product Specification Host Rabbit Antigen MCM3 Synonyms DNA polymerase alpha holoenzyme associated protein P1,P1 MCM3,RLF subunit beta,p102 Immunogen Synthetic Peptide Location Nucleus, Chromosome, Intracellular Accession P25205 Clone Number SDT 048 10 Antibody Type Rabbit mAb Application WB, IHC P, ICC, ICFCM, IP Reactivity Hu Predicted Reactivity Ms, Av, Or, Bv Purification Protein A Concentration 0. 5mg ml Conjugation Unconjugated Physical
Product Specification
| Host | Rabbit |
| Antigen | MCM3 |
| Synonyms | DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus, Chromosome, Intracellular |
| Accession | P25205 |
| Clone Number | SDT-048-10 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P, ICC, ICFCM, IP |
| Reactivity | Hu |
| Predicted Reactivity | Ms, Av, Or, Bv |
| Purification | Protein A |
| Concentration | 0.5mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1:500 | |
| ICC | 1:500 | |
| IHC-P | 1: 2000 | |
| IP | 1:25 | |
| WB | 1:2000 |
Background
DNA replication licensing factor MCM3 is a protein that in humans is encoded by the MCM3 gene. The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
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