SKU: 82252179712

Human IL-12p70 High Sensitivity ELISA Kit

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Description

Human IL-12p70 High Sensitivity ELISA KitProduct Specification Species Reactivity Human Synonym CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL 12 p70, IL 12 subunit p35, IL 12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin 12 alpha chain, interleukin 12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain

Product Specification

Species Reactivity Human
Synonym CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35
Description

Detection principle:

This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Human IL-12 capture antibody Pre-coated on high affinity enzyme plates. The standard substance, the sample to be measured and the biotin-labeled detection antibody are sequentially added into the wells of the enzyme label plate, thoroughly shaken and mixed, and then placed at room temperature for time 2 Hours of incubation process, present in the sample IL-12 Binds to solid phase antibodies and detection antibodies. After sufficient washing to remove free and unbound components, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-12 The concentration of is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.

 

Test Type: Double antibody sandwich method

 

Form: Pre-coating 96 Orifice plate

 

Test Sample Type: Cell supernatant, serum, plasma

 

Sample Load: 100 μl

 

Kit Components: Pre-coating 96 Well plates, standards, IL-12p70 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.

 

Sensitivity: 0.04 pg/mL

 

Detection range: 0.39-25 pg/mL

 

Recovery Range: 91-104%

 

Save Method: 2-8℃

 

Standard graph:

 

 

Background:

Interleukin 12(IL-12) Composed of dendritic cells, macrophages, neutrophils and human B Lymphoblastic cell line (NC-37) Arises naturally in response to antigenic stimulation. IL-12 By 4 1 а Helix composed, is a composed of 2 Species independent gene ---IL-12A(p35) And IL-12B(p40) Encoding the formed heterodimeric cytokine. Biologically active heterodimers can be formed after protein synthesis (p70) And homodimers p40 。 IL-12 Participate in initial T Cells to Th1 Process of cell differentiation, as T Cell stimulating factor, which stimulates T The growth of cells and their functions. IL-12 Has anti-angiogenic activity, and is effective against natural killer cells and T Lymphocyte activity plays an important role. Similarly, IL-12 Associated with autoimmunity.

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SKU: 82252179712

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J
Alexandria, US
★★★★★ 5
Exactly as expected
Color: Black, Size: Medium (8.5-14''W x 12''D), Color: Black, Size: Medium (8.5-14''W x 12''D)
This expandable silverware organizer made such a difference in my kitchen drawers. It sounds dramatic for a utensil organizer, but it genuinely made everything feel cleaner, more organized, and less chaotic. I love that it expands because it made it super easy to customize to my drawer size instead of awkwardly leaving unused space. The compartments are roomy enough to hold a lot without everything getting jumbled together, and I like that there are enough sections for silverware plus extra kitchen tools and gadgets. It helped me finally give everything a designated place, which is especially satisfying when setting up a new kitchen. Even though it’s plastic, it doesn’t feel flimsy or cheap. It feels sturdy once in the drawer, slides open smoothly, and has held up really well with daily use. I also appreciate that it’s lightweight and easy to wipe clean if crumbs or dust get inside. Overall, this is one of those simple organizational purchases that makes everyday life easier. My kitchen drawers look so much more put together now, and it’s nice not having to dig through clutter every time I need a fork or cooking utensil. Definitely worth it for anyone trying to organize their kitchen without spending a ton.
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Reviewed in the United States on May 21, 2026
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judy knapp
Alexandria, US
★★★★★ 5
Expandable drawer organizer
Color: Gray, Size: XL (13-22''W x 15''D)
Wonderful addition to the silverware drawer. It expands to fit the size of your drawer. It is well designed and well made. We are very pleased!
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Reviewed in the United States on May 24, 2026
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christine
Whiting, US
★★★★★ 5
Worth the money
Color: Black, Size: Medium (8.5-14''W x 12''D)
Made well and fits nicely is drawer
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Reviewed in the United States on May 27, 2026
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Boise, US
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H Salib
New York, US
★★★★★ 5
Excellent Value.
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