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Description
Human IL-12p70 High Sensitivity ELISA KitProduct Specification Species Reactivity Human Synonym CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL 12 p70, IL 12 subunit p35, IL 12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin 12 alpha chain, interleukin 12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain
Product Specification
| Species Reactivity | Human |
| Synonym | CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35 |
| Description |
Detection principle: This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Human IL-12 capture antibody Pre-coated on high affinity enzyme plates. The standard substance, the sample to be measured and the biotin-labeled detection antibody are sequentially added into the wells of the enzyme label plate, thoroughly shaken and mixed, and then placed at room temperature for time 2 Hours of incubation process, present in the sample IL-12 Binds to solid phase antibodies and detection antibodies. After sufficient washing to remove free and unbound components, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP , SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-12 The concentration of is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.
Test Type: Double antibody sandwich method
Form: Pre-coating 96 Orifice plate
Test Sample Type: Cell supernatant, serum, plasma
Sample Load: 100 μl
Kit Components: Pre-coating 96 Well plates, standards, IL-12p70 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.
Sensitivity: 0.04 pg/mL
Detection range: 0.39-25 pg/mL
Recovery Range: 91-104%
Save Method: 2-8℃
Standard graph:
Background: Interleukin 12(IL-12) Composed of dendritic cells, macrophages, neutrophils and human B Lymphoblastic cell line (NC-37) Arises naturally in response to antigenic stimulation. IL-12 By 4 1 а Helix composed, is a composed of 2 Species independent gene ---IL-12A(p35) And IL-12B(p40) Encoding the formed heterodimeric cytokine. Biologically active heterodimers can be formed after protein synthesis (p70) And homodimers p40 。 IL-12 Participate in initial T Cells to Th1 Process of cell differentiation, as T Cell stimulating factor, which stimulates T The growth of cells and their functions. IL-12 Has anti-angiogenic activity, and is effective against natural killer cells and T Lymphocyte activity plays an important role. Similarly, IL-12 Associated with autoimmunity. |
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