Human Granzyme B ELISA Kit

Detection Principle:

Granzyme B, a member of the serine protease granzyme family, mainly exists in the granules of cytotoxic T lymphocytes (CTLs) and natural killer cells (NK). Granzyme B plays an important role in particle mediated apoptosis by using substrates such as caspase 3, caspase 8 and bid

As one of the five granzymes (a, B, h, K, and m) identified in the human genome, Granzyme B (32kDa) has been most extensively studied for its biological functions and uses in health and disease. It was synthesized as a precursor (247 residues), a signal peptide (residues 1-18), a propeptide (residues 19-20), and a mature chain (residues 21-247). Once in the particle, Granzyme B is fully processed into the mature chain, and granzyme B becomes an active protease when the current peptide Gly Glu is removed from the N terminus by cathepsin C cleavage. The protease activity of granzyme B is tightly controlled by serpin b9/ protease inhibitor 9

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, Granzyme B detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 3.54 pg/ml

Detection Range: 15.63 - 1000 pg/ml

Recovery Range: 83-104%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Granzyme B is a member of the granzyme family of serine proteases, which mainly exists in cytotoxic T lymphocytes (CTLs) and natural killer cells (NK)Of the particles. Granzyme B plays an important role in particle mediated apoptosis by using substrates such as caspase 3, caspase 8 and bid

As one of the five granzymes (a, B, h, K, and m) identified in the human genome, Granzyme B (32kDa) has been most extensively studied for its biological functions and uses in health and disease. It was synthesized as a precursor (247 residues), a signal peptide (residues 1-18), a propeptide (residues 19-20), and a mature chain (residues 21-247). Once in the particle, Granzyme B is fully processed into the mature chain, and granzyme B becomes an active protease when the current peptide Gly Glu is removed from the N terminus by cathepsin C cleavage. The protease activity of granzyme B is tightly controlled by serpin b9/ protease inhibitor 9.