Human IgE ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA detection technology. Specific anti human IgE capture antibody was pre coated on a high affinity microplate. Add the standard, detection sample and horseradish peroxidase labeled detection antibody into the wells of enzyme plate. After incubation, the IgE in the sample binds to the solid-phase antibody and detection antibody. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color reaction is directly proportional to the concentration of IgE in the sample. The reaction was stopped by adding stop solution, and the absorbance value was measured at 450 nm wavelength (correction wavelength 570 - 630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, HRP labeled IgE detection antibody, detection buffer, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.

Sensitivity: 0.34 ng/ml

Detection Range: 7.81-500 ng/ml

Recovery Range: 88-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Immunoglobulin E (IGE)They are a class of antibodies that exist only in mammals. The main function of IgE is to produce immunity to parasites, such as worms, Schistosoma mansoni, Trichinella spiralis and Fasciola hepatica. IgE plays a key role in type I hypersensitivity reactions, such as allergic asthma, most sinusitis, allergic rhinitis, food allergy, chronic urticaria and atopic dermatitis. IgE also plays an important role in the response to allergens, such as allergens, bee stings, antigens used in desensitization immunotherapy. Although IgE content is the least (IgE content in normal human serum only accounts for 0.05% of all Ig classes), it can trigger the strongest inflammatory response. The content of IgE in the blood of patients with hereditary allergy is 10 times higher than that of normal people. In a variety of autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and psoriasis, IgE levels have increased. It is presumed that IgE plays an important pathogenic role in RA and SLE by inducing hypersensitivity.