SKU: 29414995514

UniOne® TR-FRET Human Epigeneous Bromodomain Binding Kit

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Description

UniOne® TR-FRET Human Epigeneous Bromodomain Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes Homogeneous Time Resolved Fluorescence(TR FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide. This method enables simple and rapidhigh throughput screening of inhibitors and blocking antibodies. As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1 21) peptide is detected using a Eu labeled Anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

This kit utilizes Homogeneous Time-Resolved Fluorescence(TR-FRET) technology to measure the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide. This method enables simple and rapidhigh-throughput screening of inhibitors and blocking antibodies.

As illustrated, the interaction between BRD4 and the[LYS(5,8,12,16)AC] H4(1-21) peptide is detected using a Eu-labeled Anti-Tag1antibody (TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRETacceptor). The binding of BRD4 to the [LYS(5,8,12,16)AC] H4(1-21) peptidebrings the donor and acceptor antibodies into close proximity. Excitation ofthe donor antibody then triggers Fluorescence Resonance Energy Transfer (FRET)to the acceptor antibody, resulting in a specific emission signal at 665 nm.The intensity of this specific signal is proportional to the degree ofinteraction between BRD4 and the [LYS(5,8,12,16)AC] H4(1-21) peptide. Thishomogeneous assay is simple to perform and requires no washing steps.



Components

Table 1. Kit Components and Storage Conditions

Component

Concentration

100 TESTS

500 TESTS

2500 TESTS

10000 TESTS

Storage Temp.

(+)-JQ-1

300μM

3μL

10μL

50μL

200μL

-80℃

Tag1-BRD4 protein

100×

4μL

20μL

100μL

400μL

-80℃

Tag2- peptide

100×

4μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu dye antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃

* Avoid repeated freeze-thaw cycles of reagents. Aliquot upon first thaw and store according to the specified conditions. Shelf life is one year.

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SKU: 29414995514

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Diane
Chelsea, US
★★★★★ 5
exceeded expectations
Format: Paperback
I received this comic book within 10 days of submitting my order. I'm very pleased with the condition of the book - It looks like new! The seller was very accurate with the description. I'll be a repeat customer! It's entertaining reading material for a hard-to-buy-for middle schooler!
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Reviewed in the United States on February 25, 2011
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Amazon Customer
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★★★★★ 5
Five Stars
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Great Simpsons pass time entertainment
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Reviewed in the United States on June 8, 2016
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Karla Tipton
Houston, US
★★★★★ 5
Accessible and insightful guide to project management
Format: Kindle
"Project Management Mastery" describes the science and art of project management in a highly accessible and insightful way. This guide is written in understandable language for beginners and can also be a refresher for seasoned project managers. The guide fully describes the concepts of planning, execution, monitoring and control, and closure. Each chapter layers new information upon the foundations of the previous chapters by addressing basic concepts and building on details. This book uses project management terminology, but defines it so the concepts are clear, not fuzzy. Also discussed in depth is the importance of team members' responsibilities and accountability, and the importance of team motivation. There is a detailed exploration of the differences between various project management methodologies, such as Agile, Waterfall, SCRUM, Kanban and other models. Also described is the methodology of PRINCE2, used primarily in the UK and Europe, as well as hybrid project management models. There is guidance on which methodology works best for the project being planned. The guide also addresses the impacts and benefits of how using AI might impact the completion of routine tasks in the project management lifecycle. Also included is a comprehensive list of references at the end, for those wanting to dive deeper into the subject. Overall, this is a great guide for new project managers, as well as a solid refresher for those reentering the field, or looking for an updated perspective.
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Reviewed in the United States on June 24, 2023
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James R. Massoni
Natrona Heights, US
★★★★★ 5
Mastering Project Management
Format: Kindle, Format: Kindle
What a wonderful book on project management. The author describes what project management is, how to create a project plan, and the different phases that exist. He covers traditional project management and newer versions of it that have evolved, such as Waterfall, Agile, Scrum, and PRINCE2. The author explains the various types of project management, what are the important tenets of each and which is best for different types of projects. He goes into a lot of detail into each phase, different software you can use, along with visualization tools available like Gantt Charts and Kanban boards. It is a good comprehensive book that goes in to detail into each area of project management, what you should do to be successful and the various places that project managers typically fail. Finally, he covers final deliverables, project closer and doing a project review and releasing resources. I thought it was a very good book, well done and one that any project manager would love to have as a resource.
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Reviewed in the United States on August 24, 2023
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Jeanna Read
Birmingham, US
★★★★★ 5
For The Seasoned Project Manager and the Beginner
Format: Kindle, Format: Kindle
It takes a talented writer to write for the expert and the beginner in the same book. But that is how Mr. Hayden writes in this book. It is obvious he is an experienced Project Manager, and he not only gives advice, but gives you an overview of project management in general, and the foundations of it. But then he jumps right into the meat of the matter - and lays out so many ways to start the project, including so many methodologies to use. He includes the SWOT method, that I used with my teams, but there are so many more that I could have used! I love the way he lays out a plan for you to use - he doesn’t just give you ideas, he lays it out in a way that you can implement it successfully, and more importantly, confidently. That is so important when leading a team! I highly recommend this book to any level of project manager! And not just those in a project manager position - also for those professionals who are leading a project even though they are in other roles in their company.
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Reviewed in the United States on October 10, 2023

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