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Description
Caspase 4 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 4 enzyme activity: 1. Take out an appropriate amount of Ac-LEVD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-LEVD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-LEVD-pNA (2mM). Add Ac-LEVD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 4 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 4, it can catalyze the substrate Leu Glu Val Asp p nitroanilide (Ac LEVD pNA) to produce yellow pNA (p-nitroaniline), which can be used to detect the activity of Caspase 4 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 4 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | caspase-4,caspase 4 |
| Description | caspase 4 Activity Detection Kit is a kit that detects caspase 4 enzyme activity or purified caspase 4 enzyme activity in cell or tissue lysates by spectrophotometry. |
| Storage Temp. | When stored at-20 °C, Ac-LEVD-pNA and pNA need to be stored in the dark. |
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4.3 ★★★★★
Based on 21 reviews
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Product Reviews
★★★★★ 5
It's Working
Scent: Smoky coffee, Size: 4 Fl Oz (Pack of 1)
I like how it softens and protect hair. I am using sparingly on my scalp and have not used enough to see hair growth yet. I have had it for a month but do not use daily. I am growing my grey hair out and I have noticed that some of the white hairs are more salt and pepper now so it naturally darkens hair. My friend who told me about the product has been using it longer and her grey hairs are pretty much unnoticed by the natural darkening effect, blending enough to give her a light brown look. I have extremely fine thin fragile hair so I leave it on instead of rinsing or washing it out per instructions, that is another reason I do not use daily on hair and scalp. So far I have not had any negative side effects by using sparingly and leaving in until weekly wash. There is a strong smokey smell but not overwhelming for me and the scent dicipates within an hour or less.so I am not bothered by it. After using product on my hair, I rub a little on my nails & cuticles. It appears to help, as my cuticles are a lot healthier now.
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Reviewed in the United States on February 1, 2024
★★★★★ 4
Ash vs. Moisture/Success?
Scent: Smoky coffee, Size: 4 Fl Oz (Pack of 1)
The product feels great on the skin and moisturizes well. Although, don't be fooled by the smokey coffee detail. I'm an avid expresso bean grinder and drinker, this is no coffee. Smells like strong chain smokers favorite corner of the house on a stressful day. Imagine ash trays and Jojoba blended. Now that that's explained, it genuinely feels great on the skin/beard/scalp. Unfortunately, I am unsure how long my wife will tolerate this as a nighttime routine.
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Reviewed in the United States on September 9, 2025
★★★★★ 5
Strong coffee smell. Might not be be compatible for everyone. However, product is really good.
Scent: Smoky coffee, Size: 4 Fl Oz (Pack of 1)
Love this product. May not be compatible for someone who doesn't like smell of coffee that does not detract from the product. it's really good for hair.
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Reviewed in the United States on January 5, 2026
★★★★★ 3
So far nothing amazing
Scent: Smoky coffee, Size: 4 Fl Oz (Pack of 1)
So I’ve been using for a couple of weeks now and I haven’t noticed any difference. I am going to continue on. I don’t know if it takes time for it to actually show some results. I will say two things about it. It comes solid for 12 my jar cracked and broken And it’s non-returnable so I just have to deal with it so that’s unfortunate after paying $20 second of all it is a solid mass so you have to put it in your hands and kind of warm it up for it to become any sort of an oil and I wouldn’t even consider it an oil at that it’s more of like a cream once you warm it up in your hands, but it doesn’t make your hair greasy. I use a small amount I would think that the jar would last a long time given that you don’t need a lot to get through to your scalp unfortunately, with my jar being cracked, I don’t know how that’s gonna affect the longevity of the product And it does have a smell think of burnt coffee beans that will dissipate after about an hour to an hour and a half after putting it on, but if you’re not putting it on, I’m just going to bed. It might be become an issue. I’m gonna wait it out. I hope I do see results from doing this but again so far nothing.
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Reviewed in the United States on February 15, 2025
★★★★★ 5
Wonderful product
Scent: Smoky coffee, Size: 4 Fl Oz (Pack of 1)
I used this on a bald spot I had and it worked extremely well. Consistency is key though it needs to be used every day
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Reviewed in the United States on April 3, 2026
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