SKU: 50608387928

Human GM-CSF ELISA Kit

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Description

Human GM-CSF ELISA KitProduct Specification Description Detection Principle: The double antibody sandwich ELISA method was used in this experiment. The anti human GM CSF monoclonal antibody is coated on the microplate, the GM CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM CSF polyclonal antibody was added, and the unbound antibody was washed away; Add

Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human GM-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.089-0.740  Pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85-104%

Storage Method: 2-8 ℃

Standard Curve

Background:

Granulocyte macrophage colony stimulating factor (GM-CSF), also known as csf-2, is a common β chain (β c)The polytrophic 30 kDa member of the cytokine family also includes IL-3 and IL-5. GM-CSF adopts &alpha with two intrachain disulfide bonds-Helical configuration. It is secreted by a variety of activated immune, mesenchymal, and epithelial cell types and circulates in the form of variable glycosylated monomers. During inflammation, it is upregulated in a variety of cell types, including encephalitic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. Mature human GM-CSF has 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively. The high affinity receptor of GM-CSF is bound by a 50 kDa ligand α Subunit (gm-csfrα)And 120 kDa signal transduction β C composition. According to reports, the stoichiometry of functional GM-CSF receptor is GM-CSF, gm-csfrα And β The 2:2:2 complex of C. It is worth noting that β The C subunit is shared by the receptor complex of IL-3 and IL-5, and IL-5 may pass through gm-csfrα And β C signaling. GM-CSF can also utilize syndecan-2 as a co receptor (14). GM-CSF has many functions. It induces the production of monocytes, neutrophils, and eosinophils from CD34 + stem cell precursors. It can synergize with IL-4 or Flt-3 ligands to induce the development and maintenance of bone marrow and dermal dendritic cells. It also functions as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. Human GM-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure the levels of human GM-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human GM-CSF expressed in E. coli and antibodies against recombinant factors. Recombinant human GM-CSF has been shown to be accurately quantified. The results obtained using natural human GM-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of human GM-CSF.

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SKU: 50608387928

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4.8 ★★★★★
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J
Verified Purchase
Jammie Clark
Fort Morgan, US
★★★★★ 4
A good read
Format: Kindle
Multiple points of view. 3 Alpha men and an Omega male. She is a Beta in training for a new program placing betas in Alpha/Omega packs. Mila is only doing the program for the money to take care of her dad. She wasn't expecting to fall for a pack but when she sees this packs Omega she is done for. There is just something about him. His Alphas are good looking as well. Too bad she is hiding a secret and their government is acting shady. I liked it and can't wait to see where their story goes.
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Reviewed in the United States on November 14, 2023
B
Verified Purchase
Bri Hires
Dallas, US
★★★★★ 3
Slightly repetitive but I did love some things
Format: Kindle
I love this type of story. And omegaverse is one of my all time favorite genres. But there are a few things that pulled me out of my enjoyment while I was reading. It was repetitive at times as well as struggled with telling not showing. So we didn’t always feel like we were experiencing things with the main character. There were also some plot holes but they may still be answered in part 2. Now this isn’t to be said I didn’t enjoy parts of the story. I loved the almost instant love between Mila and Oliver. And how he started changing around her.
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Reviewed in the United States on February 15, 2024
K
Verified Purchase
Kimberly G
Cuba, US
★★★★★ 5
delightful read
Format: Kindle
What a delightful read. The characters are awesome, the plot was so good, I loved it. I was intrigued and it kept me wanting more. Told in multiple pov, the book sucks you in and doesn’t let go. I cannot wait to read the next book.
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Reviewed in the United States on January 30, 2025
K
Verified Purchase
Kimberly B
Houston, US
★★★★★ 4
not bad
Format: Kindle
I loved the plot of this book. The characters just didn’t have a lot of depth. The connections and “love” just weren’t communicated very well in the writing. The author didn’t write the sweet psycho trope very well at all either. Lachlan was just a mess of a character.
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Reviewed in the United States on November 17, 2023
C
Verified Purchase
Carmen Alicea
Houston, US
★★★★★ 5
A Beta Worth Rooting For
Format: Kindle
In Spare, Violet Fox flips the omegaverse on its head, giving us a Beta heroine determined to make her mark. Joining the Beta Trials to support her sick father, she's thrown into a pack that doesn't want her, especially the possessive Alphas. But here's the twist: their sweet Omega turns out to be her scent match. Cue the angst, forbidden tension, and a slow-burn romance that will make your heart ache in the best way. Violet Fox delivers an emotional, refreshing take on the genre, proving Betas aren't "spares." They're stars.
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Reviewed in the United States on January 10, 2025

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