Human TPO ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human TPO capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The LH existing in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of LH in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm)

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, TPO detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 11.32pg/ml

Detection Range: 62.5-4000 pg/ml

Recovery Range: 72-124%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Thrombopoietin (TPO), also known as megakaryocyte growth and development factor (MGDF), a glycoprotein hormone produced by liver parenchymal cells, liver sinusoidal endothelial cells, and renal tubular cells. Striated muscle and bone marrow stromal cells also expressed a small amount. It can stimulate megakaryocytes to secrete and differentiate into a large number of platelets. Circulating TPO can be detected under normal and pathological conditions, including idiopathic thrombocytopenic purpura (ITP), idiopathic thrombocytosis (ET), liver cirrhosis, amegakaryocytic thrombocytopenia (AMT) and aplastic anemia (AA). The number of megakaryocytes can regulate TPO levels, while platelets can regulate plasma TPO levels. There seems to be no direct correlation between the number of platelets and blood TPO levels.