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Absin 6-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody)Product Specification References 1. Mingzhu Yang, et al., Chemical induced chromatin remodeling reprograms mouse ESCs to totipotent like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle enhanced radiotherapy synergizes with PD L1 blockade to limit post surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and
Product Specification
| References | 1. Mingzhu Yang, et al., Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. Cell Stem Cell 2022 Mar 3 Xin Guan, et al., Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nature Communication. May 20, 2022 Qian-Yue Zhang, et al., Lymphocyte infiltration and thyrocyte destruction are driven by stromal and immune cell components in Hashimoto's thyroiditis. Nature Communications Feb 9, 2022 4. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, September 2008. Ed Harlow, David Lane. Technical guidelines for antibodies (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014 70 (1): 46-58. 7. Money helps the country. Jiao Lei. Application of multi-labeled immunofluorescence staining and Doppler imaging in histological studies. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Usage | 1. Dilution of fluorescent dye: If the dye is dry powder, use 100ul DMSO to dissolve it to make 100X dye mother liquor; If the dye is liquid, it means that it is 100X mother liquor dissolved in DMSO from the factory; Use the signal amplification reaction solution to dilute at 1: 100 to prepare the dye working solution (ready-to-use); DAPI prepares the working solution using sterile water dilution at 1: 100. 2. Use of secondary antibody: This kit is matched with a universal secondary antibody for pika and rabbits, which is not suitable for mouse tissues. Please verify whether the species of the primary antibody matches before the experiment. Please refer to (# abs50030) for mouse tissue samples. 3. Frozen sections and cell crawlers must be used together with the purchase of antibody eluate (item number:abs994) |
| Experimental Procedure | https://univ.oss-cn-shanghai.aliyuncs.com/absin-china/images/experiment/20190514165729501.png |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Synonym | Multicolor kit |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, and then the next primary antibody is replaced for the second round of incubation, and another fluorescein substrate is replaced, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 520, TSA monochromatic fluorescent dye 570, TSA monochromatic fluorescent dye 620, TSA monochromatic fluorescent dye 650, TSA monochromatic fluorescent dye 700, signal amplification reaction solution, pika universal HRP labeled secondary antibody, anti-fluorescence quenching encapsulation tablet, DAPI. |
| General Notes | 1. This kit is only used for immunohistochemistry and is not used for other purposes. 2. This kit is restricted to professional use only. 3. Appropriate protective measures should be taken to avoid contact of the reagent with the skin and eyes. 4. The activity of reagents beyond the expiration date may be reduced, so reagents beyond the expiration date should not be used. 5. If the dyeing components of this kit are mixed with products of other companies, abnormalities may occur during the dyeing process. 6. Incomplete dewaxing will easily affect the dyeing effect. 7. In order to prevent possible false negative and false positive results, positive control and negative control should be carried out at the same time during the experiment. 8. All kinds of wastes generated in the use of this kit should be disposed of in accordance with the Regulations on Medical Waste Management. |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2 ~ 8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for immunohistochemical staining of tissues, paraffin sections and TMA chips. It can also be used for frozen sections and cell crawling sections, and needs to be matched with abs994 antibody eluate (specific for mIHC). |
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4.7 ★★★★★
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Product Reviews
★★★★★ 5
Super cute, well made
Color: Infinite Pink
Just what I was looking for…can’t top the cute pink
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026
★★★★★ 4
The pink is darker than the image portrays
Color: Infinite Pink
The pink color is significantly darker than the image shows. It’s like a dark red rose color in real life, not so much a hot pink like the listing shows
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Still A Fossil Fan
Color: Black, Color: Black
I have been a fan of Fossil products for many years. When I needed a new wallet I was happy to have found this style on Amazon.
I travel fairly often so I knew that I wanted a leather wallet that had a removeable hand strap, ample card storage, and a zip closure. This wallet was exactly what I was looking for.
The strap is sturdy and zipper is really smooth. The inside of the wallet is very roomy, with multiple pockets (my samsung phone fits inside). There are also two zipper sections (one interior and the other exterior). There are also several places for your ID, cards, etc.
I have been using this wallet for over a yearit now and I must say that it has held up really well. There are no pulls/ tears in the stiching or issues with the strap and the zippers still work like new.
It's a great option to have on those days when you just don't feel like carrying around a handbag. I would definitely buy this item again.
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Reviewed in the United States on October 18, 2025
★★★★★ 5
Great wallet!
Color: Brown
This was a present for my mom and she loved it!
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Reviewed in the United States on December 20, 2025
★★★★★ 5
Pretty and functional.
Color: Infinite Pink
Very well made with many compartments. Very pretty dark pink color.
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Reviewed in the United States on December 21, 2025
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