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Description
Human SSTR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a somatostatin receptor type 2 (SSTR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of somatostatin receptor type 2 (SSTR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Somatostatin receptor type 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Somatostatin receptor 2 is a protein encoded by the SSTR2 gene. The SSTR2 gene is also present in most other vertebrates. Somatostatin receptor 2 (SSTR2) belongs to the G protein-coupled receptor family and is most abundantly expressed in the pancreas (including α and β cells), but is also expressed in other tissues such as the brain and kidney, and to a lesser extent in the jejunum, colon, and liver. In the pancreas, it binds to somatostatin, inhibiting the secretion of pancreatic enzymes. During development, it stimulates neuronal migration and axonal growth. Somatostatin receptor 2 is expressed in most tumors. Patients with neuroendocrine tumors that overexpress somatostatin receptor 2 have a better prognosis. SSTR2 overexpression can be exploited to selectively deliver radioactive peptides to tumors for detection or destruction. SSTR2 also has the ability to stimulate apoptosis in many cells, including cancer cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 4
queen of Plot Twists.
Format: Kindle
Ngl, I didn’t like the way this one ended. I wonder if they’ll be a continuation or something like that. I need answers. This is the first book that I felt like I didn’t get closure from. lol. But it was a good book. And the plot twist came outta nowhere. I was reading and reading and reading and I’m like what the heck and then I was like OH DAMN! Then it just kept hitting lol. PART 2 FREIDA!!! I have questions still.
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Reviewed in the United States on May 31, 2026
★★★★★ 5
Addictive as almond M&M's
Format: Paperback
I once read a book that blew the whistle on the abuses in the system that produces young elite figure skaters and gymnasts. One mother said of another mother that she was very likeable, but she'd cut your throat behind your back. That describes a lot of Freida McFadden's heroines (and their besties) to a t.
At least one of the following always occurs in these novels.
1. If someone says, "Eh, I'm sure it's nothing," it's not. If someone believes their husband is too busy working to have an affair, he isn't. If someone suspects a knife is missing from the block, it is.
2. If a child or teen bitterly complains of being a victim of peer conflict, there's a good chance he/she is the instigator. They may even have committed or assisted in a murder in the past.
3. At some point, you will be told point blank that the character you have just spent a good chunk of the book with is the polar opposite. And even if you're familiar with the books, it may come as at least a partial surprise since this doesn't occur in most novels.
4. Everyone in the book has a secret, even if it's just hair dye.
5. There's also what I call the "Cold Case" rule: Don't argue near stairs. Ever. But I digress.
At first, happily married to a great guy Naomi Roth doesn't really protest when her husband Jeremy, after having changed the locks, insists that she go and stay at the apartment he bought for his now-deceased parents. She believes, despite a few stray doubts, that he is telling the truth when he says that it's just renovations and that it will be better this way - and he's even thoughtfully packed for her. However, soon after that, Naomi is told in no uncertain terms that Jeremy wants a divorce, which makes her a pariah with her fellow mothers, except one. Is her husband having an affair, and if so, what secrets does his mistress have? And who the heck committed the murder in the preface (there's almost one in every book, so it isn't much of a spoiler)?
As always, the author makes writing fiction look effortless, which if you've actually sat down to write some, know it usually isn't. Another addictive read.
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Reviewed in the United States on May 27, 2026
★★★★★ 4
Calling all Freida Fans!!!
Format: Kindle
4.5 stars!!!! Mrs. Freida McFadden Fans!!! This book was soooooo good!!! I started reading last night thinking I will read a few chapters before bed. Well, it is 2am 😊 so, I couldn’t put it down. All the characters are despicable…but it a good way. I had this one figured out; I did, and BAM! No I didn’t…then, twist after twist after oh s*** twist!!! Poor Ezra…sweet little Teddy….the rest, all despicable, but like I said; in such a wonderfully twisty way!!!
Naomi husband decides out of nowhere he wants a divorce. She later finds out there “is” another woman; Veronica. Their son Teddy gets shuffled back and forth from Jeremy's house to Naomi’s apartment, and……
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Heartbreaking and beautiful
Format: Kindle
This book reads like living. I was able to be Sybil, not just observe her. I found the experience of reading her story authentic and cathartic in a way. At the middle of my life it provoked a sense that’s there can be goodness and purpose in aging.
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Reviewed in the United States on May 25, 2026
★★★★★ 4
Beautifully written and unique conversational format
Format: Hardcover
This was a great read—sad, but very well done. I really enjoyed the conversational format, since I’ve never read a book told in quite that way before. It felt fresh and different, and the writing was excellent. Overall, it was a very engaging and memorable book.
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Reviewed in the United States on May 28, 2026