Human LH ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti- human lh  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The LH in the sample; Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of LH in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm)

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, LH detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 3.17 pg/ml

Detection Range: 3.17-6000pg/ml

Recovery Range: 92-118%

Storage Method: 2-8 ℃

Standard Curve:

Background:

luteinizing hormone (LH,   also known as luteinizing hormone)It is a hormone secreted by the gonadotropic cells of the anterior pituitary. LH is a heterodimeric glycoprotein, and the protein dimer contains α And &beta  2 glycopeptide subunits, and the dimers are non covalently bound without any disulfide linkage. Its structure is similar to other glycoproteins such as follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH) and human chorionic gonadotropin (hCG). In the ovary, LH induces testosterone synthesis, which is rapidly converted to estradiol. Estradiol subsequently induces LH release from the pituitary gland to promote ovulation. In the testis, LH induces the production of testosterone by Leydig cells, activating genes in Sertoli cells and thus promoting sperm formation. In addition, in the adrenal gland, LH can induce the secretion of DHEA, the precursor of sulfated androgens.