SKU: 26366196324

Human IL-33 High Sensitivity ELISA Kit

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Description

Human IL-33 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human IL 33 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 33 present in the

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-33 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-33 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-33 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-33 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.16pg/ml

Detection Range: 6.25-400 pg/ml

Recovery Range: 92-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 33 (IL-33)Also known as nf-hev and DVS 27, it is a member of the IL-1 family with a molecular weight of 30 kDa and is secreted by many types of cells, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 can induce the secretion of type II cytokines by helper T cells, mast cells, eosinophils and basophils. IL-33 activates NF - & kappa by binding to receptor ST2 and IL-1 receptor accessory protein (IL1RAP); B and extracellular molecules in the MAP kinase signaling pathway, the latter can promote polarized Th2 cells to secrete type II cytokines (such as IL-5 and IL-13), thereby exerting their biological functions. After injection of IL-33, it can induce type II cytokines in vivo, which is thought to induce severe pathological changes in mucosal organs. The ligand / receptor complex appears to signal through the JAK STAT pathway.

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SKU: 26366196324

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Phoenix, US
★★★★★ 5
Amazing product - instant smoothness
Size: 1 Fl Oz (Pack of 1)
I've only had this product for the last several days, but, so far, it's AMAZING. I've tried hyaluronic products before, because everyone says that you are supposed to for moisture, but, honestly, I was always underwhelmed. They were thin and did nothing. So, despite this one having great reviews, I was not really expecting anything, but the price was right. But this product....although liquid and clear, it's got a slight thickness to it, and spreads on very smooth. You only need a little bit to cover your entire face. Despite the slight thickness, it's not gooey or sticky at all, and it dries incredibly fast. In fact, if you don't spread it around onto your face quickly enough, it's already drying. There is no real smell to it. And, as soon as it dries onto your face, your skin feels incredibly smooth. Maybe it wouldn't if you had a significant dry skin problem to begin with, but for regular, maturing skin, it leaves skin baby smooth. And it lasts all day, and my skin looks better. I have very sensitive skin, and most things on my face eventually make my eyes water (even gentle facial moisturizers and sunscreens), but, so far, I haven't had an issue, and, because it's been a few days already, maybe I won't. Doesn't really feel like I'm wearing anything, and isn't a problem if I want to layer it with something else. I do wash it off at night, and can tell that something is still on my face even at the end of the day because it's a little slippery when I wash it. So it has staying power. For the price, I would definitely consider trying this product for moisturizing, and instantly smoother and softer skin.
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Reviewed in the United States on April 3, 2026
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Amazon Customer
West Palm Beach, US
★★★★★ 5
plumping hydration with no greasiness or oiliness
Size: 1 Fl Oz (Pack of 1)
Its featherweight and melds with the skin in an instant. It plumps without any residue or "slip" on the skin. I have normal skin that tends toward slight dehydration and I use adapalene and the Ordinary 2% Salicylic acid anhydrous for my sebaceous hyperplasia. Because of the sebaceous hyperplasia and the heat and extreme humidity where I live, I cannot use anything with oils or dimethicone or anything even remotely occlusive. This serum is a great way for me to get appropriate hydration without exacerbating those enlarged oil glands or making my face sticky and oily. I dont need my face sealed, a humectant like this pulls plenty of hydration into my skin and I dont lose it to the air because of the ambient humidity. Its pretty perfect for me. It also has kept my barrier intact while alternating the adapalene and salicylic acid. Its a huge bang for the buck, I mean the price point is unbeatable.
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Reviewed in the United States on May 11, 2026
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Amazon Customer
Dallas, US
★★★★★ 5
Great lights
Size: 2PCS 23''x5''
Very nice lights. They look really cool, and were well packaged. If you are looking for a fun accessory for your vehicle that makes people do a double take... then this is for you!
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Reviewed in the United States on May 26, 2026
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MDurocher
Los Angeles, US
★★★★★ 5
Fun
Size: 11''x5''
Works great
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Reviewed in the United States on June 1, 2026
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Grantham, US
★★★★★ 5
It's great for food delivery and driver jobs
Size: 7''x3''
My husband got this for food deliveries and uber/Lyft. The app let him add several texts. I believe he added 4. He was able to design it how he wanted it. It seemed fairly easy and I liked how it looked. The words would scroll over, like... Uber eats, etc... It included a big roll of strong red tape. The led screen product is charged by wall plug, phone, or the car usb phone charger. I think it was a spectacular product. I thought it was limited to 4 letters since it is small in size.
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Reviewed in the United States on April 20, 2026

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