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Description
Caspase 2 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 2 enzyme activity: 1. Take out an appropriate amount of Ac-VDQQD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-VDQQD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-VDQQD-pNA (2mM). Add Ac-VDQQD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 2 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on the caspase 2 specific hydrolysis of peptide substrate Ac-VDQQD-pNA (Val Asp Val Ala Asp p nitroanilide), the released free nitroaniline pNA has a maximum absorbance at 405nm. Thus, the activity of caspase 2 can be detected by absorbance. Suitable for detecting Caspase-2 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | ch-1,Nedd-2,caspase-2,caspase 2 |
| Detection Type | Mammalian tissues, cells |
| Description | Caspase 2 activity detection kit is a kit that detects caspase 2 enzyme activity or purified caspase 2 enzyme activity in cell or tissue lysates by spectrophotometry. Caspases are a family of proteases that play an important role in the process of apoptosis. Caspase 2 can be activated during signal transduction of apoptosis. Caspase 2 mRNA can be cleaved into two different length forms. The full-length mRNA product of Caspase 2 can promote apoptosis, while the short mRNA protein product can inhibit apoptosis. In vitro, caspase 2 can be activated by caspase 1, caspase 3, and granzyme B. |
| Storage Temp. | When stored at-20 °C, Ac-VDQQD-pNA and pNA need to be stored in the dark. |
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4.2 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Nice & comfortable
Size: XX-Large, Color: Grey Heather, Size: XX-Large, Color: Grey Heather
This is a nice & comfortable sweat shirt. I wear a 2X and this fits me comfortably. The stitching is right on as there are no loose threads anywhere on the shirt. This shirt is medium thickness & has a little bit of stretch to it, which is all one needs. From underwear to sweatshirts FOTL has does a great job once again. For the price paid you are getting a great value for your money.
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Reviewed in the United States on March 23, 2026
★★★★★ 3
Nice fit and warm, but bad material.
Size: 4X-Large, Color: Black
Not good material, now it looks like having four or five years of use and is not 1 year old.
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Reviewed in the United States on May 19, 2026
★★★★★ 5
Meets the demands.
Size: XX-Large, Color: Black Heather Pullover
Good quality and soft. Not to thick and breathable. Comfortable for all day use. Pilling is an issue inside after a few washes. Great value for a hoodie. Size is good but will shrink after a couple washes. Not to warm.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Excellent quality, thick, warm and comfortable!
Size: 3X-Large, Color: Duffle Bag Green
I bought this sweat shirt for my husband, and had to buy him another because I love it so much!! My children have tried to take it from me several times. It is so soft, super thick, and warm. I love the fit, it is a little baggie just how I like them. The quality is great for the money, at this price sweat shirts are usually see through and bad quality but NOT this one!!!!
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Reviewed in the United States on February 5, 2026
★★★★★ 5
Cool
Size: Small, Color: Warm Mocha
A sweater is the ultimate clothing item for staying warm and cozy. Whether it's a chilly winter day or a cool summer evening, a sweater is there to provide comfort and protection. Its soft and knitted fabric feels gentle against the skin, making it a pleasure to wear.
One of the greatest advantages of a sweater is its versatility. It can be dressed up or down, depending on the occasion. Pair it with jeans for a casual look, or wear it over a dress for a more elegant ensemble. With so many colors, patterns, and styles to choose from, there's a sweater for every taste and preference.
Not only does a sweater keep you warm, but it also adds a touch of style to your outfit. From chunky cable-knit sweaters to sleek and form-fitting designs, there's a wide range of options to suit any fashion sense. Sweaters can be accessorized with scarves, hats, and jewelry to create a unique and personalized look.
Another benefit of a sweater is its durability. Well-made sweaters are built to last, making them a wise investment in your wardrobe. With proper care, a sweater can withstand years of wear and still maintain its shape and quality.
In addition to its practicality and style, a sweater also holds sentimental value. It can remind us of cherished memories, like cozy evenings by the fireplace or family gatherings during the holidays. Wearing a favorite sweater can evoke feelings of comfort, nostalgia, and a sense of belonging.
In conclusion, a sweater is the optimal choice for both fashion and function. It keeps us warm, adds a stylish touch to our outfits, and holds sentimental value. So, next time you're getting dressed, don't forget to reach for that trusty sweater! Stay cozy, my friend! 😊🧥
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Reviewed in the United States on December 30, 2023
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