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Description
HLA-DRB1 Recombinant Rabbit mAb (SDT-R253)Product Specification Host Rabbit Antigen HLA DRB1 Synonyms Human leukocyte antigen DRB1 (HLA DRB1) Location Cell membrane, Endoplasmic reticulum membrane Accession P01911 Clone Number SDT R253 Antibody Type Recombinant mAb Application WB, IHC P, ICC Reactivity Hu Purification Protein A Concentration 0. 25 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300 Stability & Storage 12
Product Specification
| Host | Rabbit |
| Antigen | HLA-DRB1 |
| Synonyms | Human leukocyte antigen DRB1 (HLA-DRB1) |
| Location | Cell membrane, Endoplasmic reticulum membrane |
| Accession | P01911 |
| Clone Number | SDT-R253 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500 | null |
| IHC | 1:4000 | null |
| ICC | 1:250 | null |
Background
HLA class II histocompatibility antigen, DRB1 beta chain is a protein that in humans is encoded by the HLA-DRB1 gene. DRB1 encodes the most prevalent beta subunit of HLA-DR. DRB1 alleles, especially those encoding amino acid sequence changes at positions 11 and 13, are associated risk of rheumatoid arthritis. The protein encoded by this gene belongs to the HLA class II beta chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins to T helper cells. Class II molecules are constitutively expressed in professional antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages), and could be induced in non-professional APCs. Mainly used for leukemia immunophenotyping, determination of primitive cell and monocyte differentiation.
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